Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.12 extracted from

  • Zaffagnini, M.; Michelet, L.; Marchand, C.; Sparla, F.; Decottignies, P.; Le Marechal, P.; Miginiac-Maslow, M.; Noctor, G.; Trost, P.; Lemaire, S.D.
    The thioredoxin-independent isoform of chloroplastic glyceraldehyde-3-phosphate dehydrogenase is selectively regulated by glutathionylation (2007), FEBS J., 274, 212-226.
    View publication on PubMed
Search for more literature for 1.2.1.12

Cloned(Commentary)

Cloned (Comment) Organism
for expression in Escherichia coli BL21DE Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C153S mutant, having only one cysteine in the catalytic site, is oxidized and glutathionylated similarly to the wild-type enzyme Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
CuCl2 1 mM Arabidopsis thaliana
Diamide 1 mM Arabidopsis thaliana
glutathione 5 mM oxidized glutathione, GSSG, formation of mixed disulfide between glutathione and A4-GAPDH results in the inhibition of enzyme activity Arabidopsis thaliana
glutathione formation of mixed disulfide between glutathione and GAPDH results in the inhibition of enzyme activity Spinacia oleracea
H2O2 1 mM Arabidopsis thaliana
H2O2
-
 Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Spinacia oleracea 9507
-
chloroplast
-
 Arabidopsis thaliana 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36140
-
 theoretical mass of the A subunit Spinacia oleracea
39360
-
 theoretical mass of the B subunit Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-phospho-D-glyceroyl phosphate + NADH + H+ Spinacia oleracea
-
 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 ?
3-phospho-D-glyceroyl phosphate + NADH + H+ Arabidopsis thaliana
-
 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Spinacia oleracea
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glutathionylation
-
 Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana
native GAPDH is purified from spinach chloroplasts Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phospho-D-glyceroyl phosphate + NADH + H+
-
 Spinacia oleracea D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 ?
3-phospho-D-glyceroyl phosphate + NADH + H+
-
 Arabidopsis thaliana D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 ?

Subunits

Subunits Comment Organism
heterotetramer A2B2-GAPDH, light conformation, B subunits are almost identical to A subunits, expect for the presence of a C-terminal extension containing a pair of cysteines, which is the target of TRX regulation Spinacia oleracea
homotetramer A4-GAPDH Arabidopsis thaliana
multimer A8B8-GAPDH, dark conformation, B subunits are almost identical to A subunits, expect for the presence of a C-terminal extension containing a pair of cysteines, which is the target of TRX regulation Spinacia oleracea

Synonyms

Synonyms Comment Organism
A4-GAPDH
-
 Arabidopsis thaliana
A4-glyceraldehyde-3-phosphate dehydrogenase
-
 Arabidopsis thaliana
AnBn-GAPDH
-
 Spinacia oleracea
GAPDH
-
 Spinacia oleracea
GAPDH
-
 Arabidopsis thaliana
glyceraldehyde-3-phosphate dehydrogenase
-
 Spinacia oleracea
glyceraldehyde-3-phosphate dehydrogenase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 activity assay Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 activity assay Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Spinacia oleracea
NADH
-
 Arabidopsis thaliana
NADPH
-
 Spinacia oleracea