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Literature summary for 1.2.1.12 extracted from

  • Heinz, F.; Kulbe, K.D.
    Glyceraldehydephosphate dehydrogenase from liver. I. Isolation and characterization of the bovine liver enzyme (1970), Hoppe-Seyler's Z. Physiol. Chem., 351, 249-262.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2,3-Dimercaptopropanol poor activation Bos taurus
2-mercaptoethanol 10 mM, 2fold activation of the oxidation of D-glyceraldehyde 3-phosphate Bos taurus
Cys 10 mM, 2.8fold activation of the oxidation of D-glyceraldehyde 3-phosphate Bos taurus
dithioerythritol 10 mM, 2fold activation of the oxidation of D-glyceraldehyde 3-phosphate Bos taurus
EDTA poor activation Bos taurus
GSH poor activation Bos taurus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystalline enzyme does not contain NAD+ Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
PCMB
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
NAD+ pH 8.9 Bos taurus
0.19
-
NAD+ pH 8.0 Bos taurus
0.24
-
D-glyceraldehyde 3-phosphate pH 8.0 Bos taurus
0.385
-
D-glyceraldehyde 3-phosphate pH 8.9 Bos taurus
1.54
-
arsenate pH 8.9 Bos taurus
1.7
-
arsenate pH 8.0 Bos taurus
2.9
-
D-glyceraldehyde 3-phosphate pH 8.0 Bos taurus
3.4
-
phosphate pH 8.9 Bos taurus
7.15
-
phosphate pH 8.0 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
142000
-
gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50.5
-
-
Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phospho-D-glyceroyl phosphate + NADH
-
Bos taurus D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
r
D-glyceraldehyde 3-phosphate + arsenate + NAD+
-
Bos taurus 3-phospho-D-glyceroyl arsenate + NADH
-
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Bos taurus 3-phospho-D-glyceroyl phosphate + NADH
-
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Bos taurus 3-phospho-D-glyceroyl phosphate + NADH + H+
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
reduction of 3-phospho-D-glyceroyl phosphate, triethanolamine buffer Bos taurus
7.4 8.1 reduction of 1,3-diphosphogycerate, Tris buffer Bos taurus
7.8 8.1 oxidation of D-glyceraldehyde 3-phosphate, Tris buffer and triethanolamine buffer Bos taurus
8.5
-
oxidation of D-glyceraldehyde 3-phosphate, glycine buffer Bos taurus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bos taurus
NADH cofactor Bos taurus