Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2,3-Dimercaptopropanol | poor activation | Bos taurus | |
2-mercaptoethanol | 10 mM, 2fold activation of the oxidation of D-glyceraldehyde 3-phosphate | Bos taurus | |
Cys | 10 mM, 2.8fold activation of the oxidation of D-glyceraldehyde 3-phosphate | Bos taurus | |
dithioerythritol | 10 mM, 2fold activation of the oxidation of D-glyceraldehyde 3-phosphate | Bos taurus | |
EDTA | poor activation | Bos taurus | |
GSH | poor activation | Bos taurus |
Crystallization (Comment) | Organism |
---|---|
crystalline enzyme does not contain NAD+ | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PCMB | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
NAD+ | pH 8.9 | Bos taurus | |
0.19 | - |
NAD+ | pH 8.0 | Bos taurus | |
0.24 | - |
D-glyceraldehyde 3-phosphate | pH 8.0 | Bos taurus | |
0.385 | - |
D-glyceraldehyde 3-phosphate | pH 8.9 | Bos taurus | |
1.54 | - |
arsenate | pH 8.9 | Bos taurus | |
1.7 | - |
arsenate | pH 8.0 | Bos taurus | |
2.9 | - |
D-glyceraldehyde 3-phosphate | pH 8.0 | Bos taurus | |
3.4 | - |
phosphate | pH 8.9 | Bos taurus | |
7.15 | - |
phosphate | pH 8.0 | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
142000 | - |
gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
50.5 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glyceroyl phosphate + NADH | - |
Bos taurus | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
r | |
D-glyceraldehyde 3-phosphate + arsenate + NAD+ | - |
Bos taurus | 3-phospho-D-glyceroyl arsenate + NADH | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Bos taurus | 3-phospho-D-glyceroyl phosphate + NADH | - |
r | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Bos taurus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reduction of 3-phospho-D-glyceroyl phosphate, triethanolamine buffer | Bos taurus |
7.4 | 8.1 | reduction of 1,3-diphosphogycerate, Tris buffer | Bos taurus |
7.8 | 8.1 | oxidation of D-glyceraldehyde 3-phosphate, Tris buffer and triethanolamine buffer | Bos taurus |
8.5 | - |
oxidation of D-glyceraldehyde 3-phosphate, glycine buffer | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bos taurus | |
NADH | cofactor | Bos taurus |