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Literature summary for 1.2.1.12 extracted from

  • Harris, J.I.; Hocking, J.D.; Runswick, M.J.; Suzuki, K.; Walker, J.E.
    D-glyceraldehyde-3-phosphate dehydrogenase. The purification and characterisation of the enzyme from the thermophiles Bacillus stearothermophilus and Thermus aquaticus (1980), Eur. J. Biochem., 108, 535-547.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
holoenzyme and apoenzyme Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
4 * 36000, SDS-PAGE Thermus aquaticus
36000
-
4 * 36000, SDS-PAGE Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Oryctolagus cuniculus
-
-
-
Thermus aquaticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus aquaticus
-
Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16
-
apoenzyme Thermus aquaticus
16.5
-
holoenzyme Thermus aquaticus
26.69
-
-
Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phospho-D-glyceroyl phosphate + NADH
-
Oryctolagus cuniculus D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
r
3-phospho-D-glyceroyl phosphate + NADH
-
Geobacillus stearothermophilus D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Thermus aquaticus 3-phospho-D-glyceroyl phosphate + NADH
-
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Oryctolagus cuniculus 3-phospho-D-glyceroyl phosphate + NADH
-
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Geobacillus stearothermophilus 3-phospho-D-glyceroyl phosphate + NADH
-
r

Subunits

Subunits Comment Organism
tetramer
-
Oryctolagus cuniculus
tetramer 4 * 36000, SDS-PAGE Thermus aquaticus
tetramer 4 * 36000, SDS-PAGE Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
58
-
pH 7.0, 20 min, 50% inactivation Oryctolagus cuniculus
75
-
pH 7.0, 20 min, 50% inactivation Geobacillus stearothermophilus
100
-
pH 7.0, 20 min, 50% inactivation Thermus aquaticus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Thermus aquaticus
NAD+
-
Oryctolagus cuniculus
NAD+ enzyme contains 4 equivalents of firmly bound NAD+ Geobacillus stearothermophilus
NADH cofactor Oryctolagus cuniculus
NADH cofactor Geobacillus stearothermophilus