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Literature summary for 1.2.1.12 extracted from

  • Katayama, N.; Hayakawa, K.; Urabe, I.; Okada, H.
    Kinetic properties of N6-(2-carboxyethyl)-NAD(H) and poly(ethylene glycol)-bound NAD(H) for alcohol, lactate, malate and glyceraldehyde-3-phosphate dehydrogenase from different organisms (1984), Enzyme Microb. Technol., 6, 538-542.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00519
-
NAD+
-
Thermus thermophilus
0.0102
-
N6-(2-carboxyethyl)-NAD+
-
Thermus thermophilus
0.0386
-
NAD+ poly(ethylene glycol)-bound Thermus thermophilus
0.0507
-
NAD+
-
Oryctolagus cuniculus
0.0674
-
N6-(2-carboxyethyl)-NAD+
-
Oryctolagus cuniculus
0.0806
-
NAD+
-
Geobacillus stearothermophilus
0.145
-
N6-(2-carboxyethyl)-NAD+
-
Geobacillus stearothermophilus
0.23
-
NAD+ poly(ethylene glycol)-bound NAD+ Geobacillus stearothermophilus
2.46
-
NAD+ poly(ethylene glycol)-bound NAD+ Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Oryctolagus cuniculus
-
-
-
Thermus thermophilus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + N6-(2-carboxyethyl)-NAD+ + phosphate
-
Thermus thermophilus 3-phospho-D-glyceroyl phosphate + N6-(2-carboxyethyl)-NADH
-
?
D-glyceraldehyde 3-phosphate + N6-(2-carboxyethyl)-NAD+ + phosphate
-
Oryctolagus cuniculus 3-phospho-D-glyceroyl phosphate + N6-(2-carboxyethyl)-NADH
-
?
D-glyceraldehyde 3-phosphate + N6-(2-carboxyethyl)-NAD+ + phosphate
-
Geobacillus stearothermophilus 3-phospho-D-glyceroyl phosphate + N6-(2-carboxyethyl)-NADH
-
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Thermus thermophilus 3-phospho-D-glyceroyl phosphate + NADH
-
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Oryctolagus cuniculus 3-phospho-D-glyceroyl phosphate + NADH
-
?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Geobacillus stearothermophilus 3-phospho-D-glyceroyl phosphate + NADH
-
?
D-glyceraldehyde 3-phosphate + poly(ethylene glycol)-bound NAD+ + phosphate
-
Thermus thermophilus 3-phospho-D-glyceroyl phosphate + poly(ethylene glycol)-bound NADH
-
?
D-glyceraldehyde 3-phosphate + poly(ethylene glycol)-bound NAD+ + phosphate
-
Oryctolagus cuniculus 3-phospho-D-glyceroyl phosphate + poly(ethylene glycol)-bound NADH
-
?
D-glyceraldehyde 3-phosphate + poly(ethylene glycol)-bound NAD+ + phosphate
-
Geobacillus stearothermophilus 3-phospho-D-glyceroyl phosphate + poly(ethylene glycol)-bound NADH
-
?

Cofactor

Cofactor Comment Organism Structure
N6-(2-carboxyethyl)-NAD+ can replace NAD+ as cofactor Thermus thermophilus
N6-(2-carboxyethyl)-NAD+ can replace NAD+ as cofactor Oryctolagus cuniculus
N6-(2-carboxyethyl)-NAD+ can replace NAD+ as cofactor Geobacillus stearothermophilus
NAD+ poly(ethylene glycol)-bound NAD+ can replace NAD+ as cofactor Thermus thermophilus
NAD+ poly(ethylene glycol)-bound NAD+ can replace NAD+ as cofactor Oryctolagus cuniculus
NAD+ poly(ethylene glycol)-bound NAD+ can replace NAD+ as cofactor Geobacillus stearothermophilus