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Literature summary for 1.2.1.12 extracted from

  • Krishnan, G.; Altekar, W.
    Characterization of a halophilic glyceraldehyde-3-phosphate dehydrogenase from the archaebacterium Haloarcula vallismortis (1990), J. Gen. Appl. Microbiol., 36, 19-32.
No PubMed abstract available
Search for more literature for 1.2.1.12

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol maximal activation at 5 mM, dependent on sulfhydryl compound Haloarcula vallismortis
GSH dependent on sulfhydryl compound Haloarcula vallismortis

General Stability

General Stability Organism
very stable in 4 M KCl. Inactivated within 12 h in 0.5 M KCl, 50 mM Tris-HCl, pH 8.0, at 25°C Haloarcula vallismortis

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4
-
 Haloarcula vallismortis
Cu2+ 1 mM CuCl2, complete inhibition Haloarcula vallismortis
HgCl2 1 mM, complete inhibition Haloarcula vallismortis
iodoacetamide 5 mM, complete inhibition Haloarcula vallismortis
PCMB 5 mM, complete inhibition Haloarcula vallismortis
pentalenolactone insensitive to Haloarcula vallismortis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
 D-glyceraldehyde 3-phosphate
-
 Haloarcula vallismortis

Metals/Ions

Metals/Ions Comment Organism Structure
K+ KCl, activity is enhanced by increasing concentrations of the salt Haloarcula vallismortis
LiCl activity is partly enhanced Haloarcula vallismortis
NaCl activity is enhanced by increasing concentrations of the salt Haloarcula vallismortis
NH4Cl activity is enhanced by increasing concentrations of the salt Haloarcula vallismortis
RbCl activity is enhanced by increasing concentrations of the salt. Optimum activity in 3 M RbCl Haloarcula vallismortis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 4 * 40000, SDS-PAGE Haloarcula vallismortis
190000
-
 gel filtration Haloarcula vallismortis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Haloarcula vallismortis key enzyme of glycolysis 3-phospho-D-glyceroyl phosphate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Haloarcula vallismortis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haloarcula vallismortis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
41.5
-
-
 Haloarcula vallismortis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Haloarcula vallismortis 3-phospho-D-glyceroyl phosphate + NADH
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+ key enzyme of glycolysis Haloarcula vallismortis 3-phospho-D-glyceroyl phosphate + NADH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 40000, SDS-PAGE Haloarcula vallismortis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 temperature of 50% inactivation increases by 15°C, when KCl concentration is increased from 1 M to 4 M or when 1 M KCl is replaced by 1 M (NH4)2SO4 Haloarcula vallismortis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
-
 Haloarcula vallismortis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Haloarcula vallismortis