Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli strain M15 | Mycobacterium tuberculosis |
heterologous expression in Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
by using the hanging-drop vapour-diffusion method, crystallization in 2 different crystal forms, diffraction data analysis suggests the presence of up to four monomers in the asymmetric unit of the orthorhombic crystal form and of one or 2 monomers in the cubic crystal form | Mycobacterium tuberculosis |
purified recombinant His-tagged enzyme, 9 mg/ml protein in 10 mM potassium phosphate buffer, pH 8.0, and 10 mM DTT, sitting drop vapour diffusion method, mixing of 500 nl of protein and of reservoir solution, the latter containing 1.6 M ammonium sulfate and 100 mM citric acid, pH 5.0, 2 days at 18°C, two different crystal forms, X-ray diffraction structure determination and analysis at 2.18-2.75 A resolution | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, about, recombinant His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
38070 | - |
the expressed and purified monomer protein construct includes the 17-amino-acid sequence RGSHHHHHHGSACELGT between the N-terminal Met and the second amino acid Gly of the native Asd sequence, the total length of this protein is 362 amino acids | Mycobacterium tuberculosis |
75000 | - |
recombinant His-tagged enzyme, gel filtration | Mycobacterium tuberculosis |
75000 | - |
homodimer in solution, determined by gel filtration | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
aspartate-4-semialdehyde + phosphate + NADP+ | Mycobacterium tuberculosis | second enzyme in the lysine/homoserine biosynthetic pathways | L-4-aspartyl phosphate + NADPH + H+ | - |
r | |
aspartate-4-semialdehyde + phosphate + NADP+ | Mycobacterium tuberculosis H37Rv | second enzyme in the lysine/homoserine biosynthetic pathways | L-4-aspartyl phosphate + NADPH + H+ | - |
r | |
L-aspartate 4-semialdehyde + phosphate + NADP+ | Mycobacterium tuberculosis | the enzyme takes part in the lysine/homoserine-biosynthetic pathway | L-4-aspartyl phosphate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene Rv3708c | - |
Mycobacterium tuberculosis | P9WNX5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WNX5 | - |
- |
Purification (Comment) | Organism |
---|---|
by using affinity chromatography and gel filtration | Mycobacterium tuberculosis |
recombinant His-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography and gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aspartate-4-semialdehyde + phosphate + NADP+ | second enzyme in the lysine/homoserine biosynthetic pathways | Mycobacterium tuberculosis | L-4-aspartyl phosphate + NADPH + H+ | - |
r | |
aspartate-4-semialdehyde + phosphate + NADP+ | second enzyme in the lysine/homoserine biosynthetic pathways | Mycobacterium tuberculosis H37Rv | L-4-aspartyl phosphate + NADPH + H+ | - |
r | |
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
Mycobacterium tuberculosis | L-4-aspartyl phosphate + NADPH + H+ | - |
? | |
L-aspartate 4-semialdehyde + phosphate + NADP+ | the enzyme takes part in the lysine/homoserine-biosynthetic pathway | Mycobacterium tuberculosis | L-4-aspartyl phosphate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, about, recombinant His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
homodimer | recombinant enzyme | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
ASADH | - |
Mycobacterium tuberculosis |
Asd | - |
Mycobacterium tuberculosis |
aspartate semialdehyde dehydrogenase | - |
Mycobacterium tuberculosis |
aspartate-beta-semialdehyde dehydrogenase | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Mycobacterium tuberculosis |