Application | Comment | Organism |
---|---|---|
drug development | ASADH is a target for the development of novel antibiotics, especially for Gram-negative bacteria that require diaminopimelate for cell-wall biosynthesis | Vibrio cholerae serotype O1 |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Vibrio cholerae serotype O1 |
Crystallization (Comment) | Organism |
---|---|
apoenzyme and enzyme in complex with substrate L-aspartate semialdehyde, method optimization, purified protein in 50 mM sodium citrate, pH 5.6, with 0.2 M ammonium acetate and 2 mM DTT via dialysis overnight, hanging drop vapour diffusion method, 4°C, diluted back into 100 mM Tris, pH 8.5, with 200 mM ammonium acetate and 5 mM DTT with 12 mg/ml protein, 0.001 ml protein solution is mixed with 0.001 ml of precipitant containing 0.1 M sodium citrate, pH 5.5-6.5, 5 mM DTT, 0.1-0.4 M ammonium acetate, and 24-27% PEG 8000, method optimization, overview, X-ray diffraction structure determination and analysis at 2.0-2.2 A resolution, molecular replacement method | Vibrio cholerae serotype O1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + phosphate + NADP+ | Vibrio cholerae serotype O1 | the essential ASADH produces the first branch-point metabolite in the biosynthetic pathways that lead to the production of lysine, threonine, methionine and isoleucine as well as the cell-wall precursor diaminopimelate | L-4-aspartyl phosphate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae serotype O1 | - |
isozymes ASADH1 and ASADH2 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli to over 95% purity by anion exchange chromatography and gel filtration | Vibrio cholerae serotype O1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
Vibrio cholerae serotype O1 | L-4-aspartyl phosphate + NADPH + H+ | - |
? | |
L-aspartate 4-semialdehyde + phosphate + NADP+ | the essential ASADH produces the first branch-point metabolite in the biosynthetic pathways that lead to the production of lysine, threonine, methionine and isoleucine as well as the cell-wall precursor diaminopimelate | Vibrio cholerae serotype O1 | L-4-aspartyl phosphate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Vibrio cholerae serotype O1 |
Synonyms | Comment | Organism |
---|---|---|
ASADH | - |
Vibrio cholerae serotype O1 |
aspartate beta-semialdehyde dehydrogenase | - |
Vibrio cholerae serotype O1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Vibrio cholerae serotype O1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Vibrio cholerae serotype O1 |