Literature summary for 1.2.1.11 extracted from

Blanco, J.; Moore, R.A.; Viola, R.E.
Capture of an intermediate in the catalytic cycle of L-aspartate-beta-semialdehyde dehydrogenase (2003), Proc. Natl. Acad. Sci. USA, 100, 12613-12617.

Application

Application	Comment	Organism
pharmacology	enzyme is a target for development of antibiotics	Haemophilus influenzae

Crystallization (Commentary)

Crystallization (Comment)	Organism
15 mg/ml purified recombinant enzyme, in 10 mM HEPES, pH 7.0, 1 mM EDTA, 1 mM DTT, crystallized as apoenzyme, as hemithioacetal, or as hemithioacetal structure with bound phosphate, hanging drop vapour diffusion method, 20°C, 1:1 mixture of protein solution and precipitant solution, the latter containing 24-28% PEG 3350, 0.2 M ammonium acetate, 0.1 M Tris, pH 8.5, overnight, substrate complexing by soaking of crystals in mother liquor with 50 mM potassium phosphate, crystals are frozen in precipitant solution with 20% glycerol added, X-ray diffraction structure determination and analysis at 2.0-2.15 A resolution, modeling	Haemophilus influenzae

Crystallization (Comment)

Organism

15 mg/ml purified recombinant enzyme, in 10 mM HEPES, pH 7.0, 1 mM EDTA, 1 mM DTT, crystallized as apoenzyme, as hemithioacetal, or as hemithioacetal structure with bound phosphate, hanging drop vapour diffusion method, 20°C, 1:1 mixture of protein solution and precipitant solution, the latter containing 24-28% PEG 3350, 0.2 M ammonium acetate, 0.1 M Tris, pH 8.5, overnight, substrate complexing by soaking of crystals in mother liquor with 50 mM potassium phosphate, crystals are frozen in precipitant solution with 20% glycerol added, X-ray diffraction structure determination and analysis at 2.0-2.15 A resolution, modeling

Haemophilus influenzae

Inhibitors

Inhibitors	Comment	Organism	Structure
S-methyl cysteine sulfoxide	inhibitor binding structure deduced from crystal structure	Haemophilus influenzae

Organism

Organism	UniProt	Comment	Textmining
Haemophilus influenzae	P44801	-	-
no activity in Homo sapiens	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH + H+	catalytic mechanism, identification and structural characterization of the tetrahedral reaction intermediate, substrate binding structure	Haemophilus influenzae	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-aspartate-4-semialdehyde + phosphate + NADP+	-	Haemophilus influenzae	L-4-aspartyl phosphate + NADPH	-	?

Synonyms

Synonyms	Comment	Organism
ASADH	-	Haemophilus influenzae
L-aspartate-beta-semialdehyde dehydrogenase	-	Haemophilus influenzae

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	dependent on	Haemophilus influenzae