Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Escherichia coli |
77500 | - |
ultracentrifugation | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-4-aspartyl phosphate + NADPH | Escherichia coli | enzyme in pathway from L-aspartic acid to L-lysine, L-methionine, L-threonine and L-isoleucine | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
mutant derepressed for aspartate-beta-semialdehyde dehydrogenase biosynthesis | - |
Purification (Comment) | Organism |
---|---|
using column chromatography on DEAE-cellulose, DEAE-Sephadex A-50, hydroxylapatite, ultrogel ACA-44 and Sepharose C3 | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
100 | 110 | - |
Escherichia coli |
105 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 10 mM potassium phosphate, pH 7.2, 0.1 mM EDTA, 1 mM dithioerythritol, 50% glycerol, 2 months | Escherichia coli |
4°C, 10 mM potassium phosphate, pH 7.2, 0.1 mM EDTA, 1 mM dithioerythritol, several days | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-4-aspartyl phosphate + NADPH | enzyme in pathway from L-aspartic acid to L-lysine, L-methionine, L-threonine and L-isoleucine | Escherichia coli | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
Escherichia coli | L-4-aspartyl phosphate + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli | |
NADPH | L-aspartate 4-semialdehyde dehydrogenase transfers the pro-S hydrogen from NADPH | Escherichia coli |