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Literature summary for 1.2.1.11 extracted from

  • Hegeman, G.D.; Cohen, G.N.; Morgan, R.
    Aspartic semialdehyde dehydrogenase (Escherichia coli K12) (1970), Methods Enzymol., 17A, 708-713.
No PubMed abstract available
Search for more literature for 1.2.1.11

General Stability

General Stability Organism
lyophilization of purified or partially purified enzyme, slight loss of activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
aromatic aldehydes e.g.: benzaldehyde, weak Escherichia coli
Borate
-
 Escherichia coli
formaldehyde
-
 Escherichia coli
Glutaraldehyde
-
 Escherichia coli
iodoacetate
-
 Escherichia coli
K+
-
 Escherichia coli
additional information not: chelating agents Escherichia coli
N-ethylmaleimide
-
 Escherichia coli
Na+
-
 Escherichia coli
NH4+
-
 Escherichia coli
p-hydroxymercuribenzoate
-
 Escherichia coli
Tris salts
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 NADP+
-
 Escherichia coli
0.2
-
 L-aspartate 4-semialdehyde
-
 Escherichia coli
6
-
 HAsO42-
-
 Escherichia coli
9
-
 phosphate
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 K-12
-

Purification (Commentary)

Purification (Comment) Organism
K-12, using ammonium sulfate precipitation and column chromatography on Sephadex G-200 and DEAE-Sephadex A-50 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
120
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
frozen or on ice, after dialysis, 1 month stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate 4-semialdehyde + HasO42- + NADP+
-
 Escherichia coli ?
-
 r
L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Escherichia coli L-4-aspartyl phosphate + NADPH
-
 ?
additional information maximum velocity with HAsO42- is 0.4 times that with HPO42- Escherichia coli ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 20 min, crude extract, 20% loss of activity Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
additional information NAD+: 0.1% or less the rate of reaction with NADP+ Escherichia coli
NADP+
-
 Escherichia coli