Application | Comment | Organism |
---|---|---|
medicine | hypochlorous acid and chloramines have the potential to inactivate KGDHC, a major target in neurodegeneration | Sus scrofa |
medicine | hypochlorous acid and chloramines have the potential to inactivate KGDHC, a major target in neurodegeneration, without significant loss of cellular viability | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | inhibits lysate KGDHC in a concentration- and time-dependent manner, inhibitory effect reversed by addition of dithiothreitol | Mus musculus | |
H2O2 | ca. 300 times less effective than mono-N-chloramine | Sus scrofa | |
hypochlorous acid | myeloperoxidase product, inhibition of the alpha-ketoglutarate dehydrogenase multienzyme complex in vivo | Homo sapiens | |
hypochlorous acid | concentration-dependent monophasic inhibition | Mus musculus | |
hypochlorous acid | inhibits at concentrations ca. 50 times less than the effective mono-N-chloramine concentrations; myeloperoxidase product, inhibition of the alpha-ketoglutarate dehydrogenase multienzyme complex in vitro | Sus scrofa | |
mono-N-chloramine | myeloperoxidase product, inhibition of the alpha-ketoglutarate dehydrogenase multienzyme complex in vivo | Homo sapiens | |
mono-N-chloramine | inhibits both lysate and in situ KGDHC activities in a concentration-dependent manner in three distinct phases | Mus musculus | |
mono-N-chloramine | inhibits in a time- and concentration-dependent manner; myeloperoxidase product, inhibition of the alpha-ketoglutarate dehydrogenase multienzyme complex in vitro | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens | |
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine | Homo sapiens | - |
[dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | - |
ir | |
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine | Sus scrofa | - |
[dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | - |
ir | |
additional information | Homo sapiens | loss of enzyme activity contributes to the major loss of cerebral energy metabolism leading to a number of neurodegenerative disorders and Alzheimer's disease, myeloperoxidase activity is involved | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Sus scrofa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | resulting in loss of enzyme complex activity | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
heart | - |
Sus scrofa | - |
microglia | - |
Homo sapiens | - |
additional information | N2a cells | Mus musculus | - |
Neuro-2a cell | neuroblastoma cells | Mus musculus | - |
neuron | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine | - |
Homo sapiens | [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | - |
ir | |
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine | - |
Sus scrofa | [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | - |
ir | |
additional information | loss of enzyme activity contributes to the major loss of cerebral energy metabolism leading to a number of neurodegenerative disorders and Alzheimer's disease, myeloperoxidase activity is involved | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-ketoglutarate dehydrogenase complex | - |
Mus musculus |
alpha-ketoglutarate dehydrogenase complex | - |
Homo sapiens |
alpha-ketoglutarate dehydrogenase complex | - |
Sus scrofa |
KGDHC | - |
Mus musculus |
KGDHC | - |
Homo sapiens |
KGDHC | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
37 | - |
in vivo assay at, N2a cells | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Sus scrofa |
7.4 | - |
in vivo assay at, N2a cells | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
NAD+ | - |
Sus scrofa | |
thiamine diphosphate | - |
Sus scrofa | |
thiamine diphosphate | thiamine deficiency results in decreased enzyme complex activity and selective neuronal loss | Homo sapiens |