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Literature summary for 1.2.1.105 extracted from

  • De Kok, A.; Kornfeld, S.; Benziman, M.; Milner, Y.
    Subunit composition and partial reactions of the 2-oxoglutarate dehydrogenase complex of Acetobacter xylinum (1980), Eur. J. Biochem., 106, 49-58.
    View publication on PubMed
Search for more literature for 1.2.1.105

Activating Compound

Activating Compound Comment Organism Structure
AMP enhances the rate of succinylation of the enzyme complex Komagataeibacter xylinus

Inhibitors

Inhibitors Comment Organism Structure
NEM prevents desuccinylation of the 2-oxoglutarate dehydrogenase complex Komagataeibacter xylinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 2600000 Da is the MW of the 2-oxoglutarate dehydrogenase complex, calculation from sedimentation and diffusion data Komagataeibacter xylinus
100000
-
 4 * 100000, active 2-oxoglutarate dehydrogenase component, SDS-PAGE Komagataeibacter xylinus
360000
-
 active 2-oxoglutarate dehydrogenase component, gel filtration Komagataeibacter xylinus

Organism

Organism UniProt Comment Textmining
Komagataeibacter xylinus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 2-oxoglutarate dehydrogenase complex consists of 3 enzymes: E1 (alpha-ketoglutarate dehydrogenase, EC 1.2.4.2), E2 (dihydrolipoyl transsuccinylase, EC 2.3.1.61), E3 (dihydrolipoyl dehydrogenase, EC 1.8.1.4) Komagataeibacter xylinus
a
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 the enzyme complex catalyzes the reaction : 2-oxoglutarate + CoA + NAD+--> succinyl-CoA + CO2 + NADH, the following partial reactions are catalyzed: 1. HOOC(CH2)2COCOOH + (thiamine diphosphate)-E1--> (HOOC(CH2)2 CHOH-thiamine-diphosphate)-E1 + CO2, 2. (HOOC(CH2)2CH OH-thiamine-diphosphate)-E1 + (LipS2)-E2--> (HOOC(CH)2 CO-(SLipSH))-E2 + (thiamine-diphosphate)-E1, 3. (HOOC(CH2)2CO-(SLipSH))-E2 + HSCoA--> (Lip(SH)2)-E2 + HOOC(CH2)2CO-SCoA, 4. (Lip(SH)2)-E2 + E3-FAD--> (LipS2)-E2 + reduced E3-FAD, 5. reduced E3-FAD + NAD+--> E3-FAD + NADH Komagataeibacter xylinus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + lipoamide
-
 Komagataeibacter xylinus S-succinyldihydrolipoamide + CO2
-
 ?

Subunits

Subunits Comment Organism
More the complex constists of 12 chains of E1, EC 1.2.4.2, 12 chains of E2, EC 2.3.1.61 and 12 chains of E3, EC 1.8.1.4 Komagataeibacter xylinus
tetramer 4 * 100000, active 2-oxoglutarate dehydrogenase component, SDS-PAGE Komagataeibacter xylinus

Cofactor

Cofactor Comment Organism Structure
additional information the complex contains 12 lipoyl groups Komagataeibacter xylinus
additional information the complex contains 12.1 mol FAD per mol of enzyme complex Komagataeibacter xylinus