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Literature summary for 1.2.1.104 extracted from

  • Igamberdiev, A.; Lernmark, U.; Gardestroem, P.
    Activity of the mitochondrial pyruvate dehydrogenase complex in plants is stimulated in the presence of malate (2014), Mitochondrion, 19, 184-190 .
    View publication on PubMed
Search for more literature for 1.2.1.104

Activating Compound

Activating Compound Comment Organism Structure
malate malate is stimulatory for the mitochondrial pyruvate dehydrogenase complex, but not for barley chloroplast pyruvate dehydrogenase complex. The stimulation is saturated below 1 mM malate and is related to the partially activated complex, which activity increases in the presence of malate by about twofold. Malate also reverses the reduction of pyruvate dehydrogenase complex activity in the presence of glycine Hordeum vulgare
malate malate is stimulatory for the mitochondrial pyruvate dehydrogenase complex, but not for barley chloroplast pyruvate dehydrogenase complex. The stimulation is saturated below 1 mM malate and is related to the partially activated complex, which activity increases in the presence of malate by about twofold. Malate also reverses the reduction of pyruvate dehydrogenase complex activity in the presence of glycine Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0132
-
 CoA absence of malate, pH 7.6, temperature not specified in the publication Hordeum vulgare
0.076
-
 NAD+ absence of malate, pH 7.6, temperature not specified in the publication Hordeum vulgare
0.123
-
 pyruvate absence of malate, pH 7.6, temperature not specified in the publication Hordeum vulgare
0.128
-
 pyruvate presence of 1 mM malate, pH 7.6, temperature not specified in the publication Hordeum vulgare
0.136
-
 NAD+ presence of 1 mM malate, pH 7.6, temperature not specified in the publication Hordeum vulgare
0.61
-
 CoA presence of 1 mM malate, pH 7.6, temperature not specified in the publication Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Hordeum vulgare 5739
-
mitochondrion
-
 Pisum sativum 5739
-

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-
Pisum sativum P52904 and P52902 and P31023 P52904 i.e. E1 component subunit beta, cf. EC 1.2.4.1, P52902 i.e. E1 component subunit alpha, cf. EC 1.2.4.1, P31023 i.e. dihydrolipoyl dehydrogenase, cf. EC 1.8.1.4
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Hordeum vulgare
-
leaf
-
 Pisum sativum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + CoA + NAD+
-
 Hordeum vulgare acetyl-CoA + CO2 + NADH
-
 ?