Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Azotobacter vinelandii |
General Stability | Organism |
---|---|
enzyme is stable in high ionic strength buffers | Azotobacter vinelandii |
enzyme is unstable in low ionic strength buffers and in the presence of organic solvents | Azotobacter vinelandii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | product inhibition, apart from interaction with the active site on subunit E2p, acetyl CoA interacts directly with the E1p component | Azotobacter vinelandii | |
CoA | allosteric inhibition | Azotobacter vinelandii |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | 1-2%, rapid inactivation | Azotobacter vinelandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | V9H1F5 | subunit E1p, cf. EC 1.2.4.1 | - |
Storage Stability | Organism |
---|---|
4°C, recombinant enzyme in saturated ammonium sulfate, pH 7.0, stable for 6 months | Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | non-complex-bound E1p shows the same cooperativity as found for complex-bound E1p. A Hill coefficient of 1.2-1.6 is calculated for non-complex bound E1p | Azotobacter vinelandii | ? | - |
- |
|
pyruvate + CoA + ferricyanide | - |
Azotobacter vinelandii | acetyl-CoA + CO2 + ferrocyanide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PdhE | - |
Azotobacter vinelandii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
15 min, 60% residual activity | Azotobacter vinelandii |