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Literature summary for 1.2.1.104 extracted from

  • Korotchkina, L.G.; Sidhu, S.; Patel, M.S.
    Characterization of testis-specific isoenzyme of human pyruvate dehydrogenase (2006), J. Biol. Chem., 281, 9688-9696.
    View publication on PubMed
Search for more literature for 1.2.1.104

Protein Variants

Protein Variants Comment Organism
S203A isoform PDH2, mutation of phosphorylation site Homo sapiens
S203A/S271A phosphorylation at remaining site S264 causes 96% inactivation Homo sapiens
S203A/S64A phosphorylation at remaining site S271 causes complete inactivation Homo sapiens
S203E isoform PDH2, mutation of phosphorylation site Homo sapiens
S264A isoform PDH2, mutation of phosphorylation site, no enzymic activity Homo sapiens
S264E isoform PDH2, mutation of phosphorylation site Homo sapiens
S271A isoform PDH2, mutation of phosphorylation site, no enzymic activity Homo sapiens
S271E isoform PDH2, mutation of phosphorylation site Homo sapiens
S64A/S271A phosphorylation at remaining site S203 causes 91% inactivation Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0226
-
 pyruvate pH 7.0, 37°C, mutant S271A of isoform PDH2 Homo sapiens
0.0258
-
 pyruvate pH 7.0, 37°C, mutant S271E of isoform PDH2 Homo sapiens
0.0263
-
 pyruvate pH 7.0, 37°C, mutant S203A of isoform PDH2 Homo sapiens
0.0359
-
 pyruvate pH 7.0, 37°C, isoform PDH2 Homo sapiens
0.0565
-
 pyruvate pH 7.0, 37°C, mutant S203E of isoform PDH2 Homo sapiens
0.0648
-
 pyruvate pH 7.0, 37°C, isoform PDH1 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 comparison of recombinant somatic cell-specific isoform PDH1 and testis-specific isoform PDH2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein regulation of activity by pyruvate dehydrogenase kinase isoenzymes. PDK2 has the highest activity for site S264 of PDH2, PDK3 has higher activity for site S271 than for site S264, and only PDK1 can phosphorylate site S203 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
testis testis-specific isoform PDH2 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24.6
-
 pH 7.0, 37°C, isoform PDH2 Homo sapiens
25.9
-
 pH 7.0, 37°C, isoform PDH1 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + CoA + NAD+
-
 Homo sapiens acetyl-CoA + CO2 + NADH
-
 ?

Subunits

Subunits Comment Organism
More the affinity of PDH2 for the PDH-binding domain of E2 of pyruvate dehydrogenase complex differs only modestly from that of PDH1, surface plasma resonance studies Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26
-
 pyruvate pH 7.0, 37°C, mutant S271E of isoform PDH2 Homo sapiens
29.2
-
 pyruvate pH 7.0, 37°C, mutant S203A of isoform PDH2 Homo sapiens
32.6
-
 pyruvate pH 7.0, 37°C, mutant S271A of isoform PDH2 Homo sapiens
38.1
-
 pyruvate pH 7.0, 37°C, mutant S203E of isoform PDH2 Homo sapiens
66.1
-
 pyruvate pH 7.0, 37°C, isoform PDH2 Homo sapiens
69.9
-
 pyruvate pH 7.0, 37°C, isoform PDH1 Homo sapiens