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Literature summary for 1.2.1.104 extracted from

  • Strumilo, S.; Czygier, M.; Kondracikowska, J.; Dobrzyn, P.; Czerniecki, J.
    Kinetic and spectral investigation of allosteric interaction of coenzymes with 2-oxo acid dehydrogenase complexes (2002), J. Mol. Struct., 614, 221-226.
No PubMed abstract available
Search for more literature for 1.2.1.104

Activating Compound

Activating Compound Comment Organism Structure
thiamine diphosphate exogenous addition activates, involved in conformational changes Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic study, reaction of pyruvate dehydrogenase complex Bos taurus
0.0002
-
 pyruvate 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in presence of thiamine diphosphate, in presence of 2 mM Mn2+ Bos taurus
0.0006
-
 pyruvate 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in presence of thiamine diphosphate, in presence of 2 mM Ca2+ Bos taurus
0.0007
-
 pyruvate 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in absence of thiamine diphosphate, in presence of 2 mM Mn2+ Bos taurus
0.0016
-
 pyruvate 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in absence of thiamine diphosphate, in presence of 2 mM Ca2+ Bos taurus
0.004
-
 CoA 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in presence of thiamine diphosphate Bos taurus
0.012
-
 CoA 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in absence of thiamine diphosphate Bos taurus
0.023
-
 pyruvate 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in presence of thiamine diphosphate, without divalent cations Bos taurus
0.033
-
 NAD+ 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in presence of thiamine diphosphate Bos taurus
0.063
-
 pyruvate 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in absence of thiamine diphosphate and divalent cations Bos taurus
0.07
-
 NAD+ 2-oxoglutarate dehydrogenase complex, pH 7.6, 30°C, in absence of thiamine diphosphate Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + CoA + NAD+ Bos taurus
-
 acetyl-CoA + CO2 + NADH
-
 ir

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 pyruvate dehydrogenase complex
-

Purification (Commentary)

Purification (Comment) Organism
pyruvate dehydrogenase complex from ox heart, by PEG fractionation, ultracentrifugation, and gel filtration Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Bos taurus
-
myocardium
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + CoA + NAD+
-
 Bos taurus acetyl-CoA + CO2 + NADH
-
 ir

Synonyms

Synonyms Comment Organism
PDC
-
 Bos taurus
pyruvate dehydrogenase complex
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bos taurus