Any feedback?
Literature summary for 1.2.1.104 extracted from
- The pyruvate dehydrogenase complex from thermophilic organisms: thermal stability and re-association from the enzyme components (1998), Biochim. Biophys. Acta, 1385, 341-352.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| GTP | 1-10 mM, slight activation | [Bacillus] caldolyticus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GTP | 5 mM, about 50% inhibition after 3 h | Geobacillus stearothermophilus | |
| MgATP2- | 5 mM, about 70% inhibition after 3 h | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
alpha2,beta2, 2 * 42000 + 2 * 36000, SDS-PAGE, calculated from gene sequence | Geobacillus stearothermophilus |
| 42000 | - |
alpha2,beta2, 2 * 42000 + 2 * 36000, SDS-PAGE, calculated from gene sequence | Geobacillus stearothermophilus |
| 100000 | - |
x * 100000, SDS-PAGE | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
| Thermus thermophilus | - |
- |
- |
| [Bacillus] caldolyticus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | not regulated by phosphorylation/dephosphorylation | Geobacillus stearothermophilus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
| pyruvate dehydrogenase can be resolved from complex by incubation with 8 M urea, 4 M guanidinium chloride, 100 mM glycine/NaOH, pH 9.0 followed by gel filtration | Thermus thermophilus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| no reconstitution of whole complex, pyruvate dehydrogenase subunit regains some activity after separation from complex in the presence of chaperonins and ATP/MgCl2 | Geobacillus stearothermophilus |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 100000, SDS-PAGE | Thermus thermophilus |
| tetramer | alpha2,beta2, 2 * 42000 + 2 * 36000, SDS-PAGE, calculated from gene sequence | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
pyruvate dehydrogenase complex, no loss of activity after 50 min | Geobacillus stearothermophilus |
| 72 | - |
pyruvate dehydrogenase, complete loss of activity | Geobacillus stearothermophilus |
| 84 | - |
pyruvate dehydrogenase | [Bacillus] caldolyticus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
