Literature summary for 1.2.1.104 extracted from

Henderson, C.E.; Perham, R.N.
Purificaton of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus and resolution of its four component polypeptides (1980), Biochem. J., 189, 161-172.

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Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
alpha and beta subunits purified from enzyme complex after gel filtration in the presence of 2 M KI followed by chromatography on hydroxyapatite in the presence of 8 M urea	Geobacillus stearothermophilus
pyruvate dehydrogenase complex composed of EC 1.2.4.1, EC 2.3.1.12, EC 1.8.1.4	Geobacillus stearothermophilus

Storage Stability

Storage Stability	Organism
-15°C, 50 mM potassium phosphate buffer, pH 7.0, 2 mM EDTA, 0.15 mM phenylmethanesulfonylfluoride, 0.2 g/l NaN3, several months	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)