BRENDA - Enzyme Database
show all sequences of 1.2.1.100

Enzymes of vitamin B6 degradation. Purification and properties of isopyridoxal dehydrogenase and 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylic-acid dehydrogenase

Lee, Y.; Nelson, M.; Snell, E.; J. Biol. Chem. 261, 15106-15111 (1986)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
NAD+
pH 8.0, temperature not specified in the publication
Pseudomonas sp. MA-1
0.1
-
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate
pH 8.0, temperature not specified in the publication
Pseudomonas sp. MA-1
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
-
Pseudomonas sp. MA-1
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas sp. MA-1
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O
-
745254
Pseudomonas sp. MA-1
3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
-
-
-
?
additional information
enzyme catalyzes with equal facility both the oxidation of formyl-3-hydroxy-2-methylpyridine-4-carboxylic-acid by NAD+ to 3-hydroxy-2-methylpyridine-4,5-dicarboxylic acid and the reduction of formyl-3-hydroxy-2-methylpyridine-4-carboxylic-acid by NADH to 4-pyridoxic acid, i.e. reaction of 4-pyridoxic acid dehydrogenase. No oxidation of 4-pyridoxic acid is observed
745254
Pseudomonas sp. MA-1
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
-
Pseudomonas sp. MA-1
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
400
670
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate
pH 8.0, temperature not specified in the publication
Pseudomonas sp. MA-1
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
8.5
-
Pseudomonas sp. MA-1
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
NAD+
pH 8.0, temperature not specified in the publication
Pseudomonas sp. MA-1
0.1
-
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate
pH 8.0, temperature not specified in the publication
Pseudomonas sp. MA-1
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
-
Pseudomonas sp. MA-1
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O
-
745254
Pseudomonas sp. MA-1
3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
-
-
-
?
additional information
enzyme catalyzes with equal facility both the oxidation of formyl-3-hydroxy-2-methylpyridine-4-carboxylic-acid by NAD+ to 3-hydroxy-2-methylpyridine-4,5-dicarboxylic acid and the reduction of formyl-3-hydroxy-2-methylpyridine-4-carboxylic-acid by NADH to 4-pyridoxic acid, i.e. reaction of 4-pyridoxic acid dehydrogenase. No oxidation of 4-pyridoxic acid is observed
745254
Pseudomonas sp. MA-1
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
-
Pseudomonas sp. MA-1
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
400
670
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate
pH 8.0, temperature not specified in the publication
Pseudomonas sp. MA-1
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
8.5
-
Pseudomonas sp. MA-1
Expression
Organism
Commentary
Expression
Pseudomonas sp. MA-1
expression is induced in response to growth on pyridoxine
up
Expression (protein specific)
Organism
Commentary
Expression
Pseudomonas sp. MA-1
expression is induced in response to growth on pyridoxine
up
Other publictions for EC 1.2.1.100
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744227
Mugo
Crystal structure of 5-formyl ...
Mesorhizobium japonicum
Biochem. Biophys. Res. Commun.
456
35-40
2015
-
-
1
1
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
745236
Yokochi
Gene identification and chara ...
Mesorhizobium loti, Mesorhizobium loti MAFF303099
J. Biochem.
145
493-503
2009
-
-
-
-
2
-
-
4
-
-
1
-
-
5
-
-
-
-
-
-
-
-
4
1
1
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
4
1
1
-
-
2
1
-
-
-
-
1
1
-
-
-
745254
Lee
Enzymes of vitamin B6 degrada ...
Pseudomonas sp. MA-1
J. Biol. Chem.
261
15106-15111
1986
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
1
-
-
-
1
-
-
1
-
-