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Literature summary for 1.2.1.100 extracted from
- Enzymes of vitamin B6 degradation. Purification and properties of isopyridoxal dehydrogenase and 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylic-acid dehydrogenase (1986), J. Biol. Chem., 261, 15106-15111 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
NAD+ | pH 8.0, temperature not specified in the publication | Pseudomonas sp. MA-1 |  |
| 0.1 | - |
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate | pH 8.0, temperature not specified in the publication | Pseudomonas sp. MA-1 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
- |
Pseudomonas sp. MA-1 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. MA-1 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O | - |
Pseudomonas sp. MA-1 | 3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+ | - |
? | |
| additional information | enzyme catalyzes with equal facility both the oxidation of formyl-3-hydroxy-2-methylpyridine-4-carboxylic-acid by NAD+ to 3-hydroxy-2-methylpyridine-4,5-dicarboxylic acid and the reduction of formyl-3-hydroxy-2-methylpyridine-4-carboxylic-acid by NADH to 4-pyridoxic acid, i.e. reaction of 4-pyridoxic acid dehydrogenase. No oxidation of 4-pyridoxic acid is observed | Pseudomonas sp. MA-1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Pseudomonas sp. MA-1 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 400 | 670 | 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate | pH 8.0, temperature not specified in the publication | Pseudomonas sp. MA-1 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8.5 | - |
Pseudomonas sp. MA-1 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Pseudomonas sp. MA-1 | expression is induced in response to growth on pyridoxine | up |
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