Gene identification and characterization of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD+-dependent dismutase
Yokochi, N.; Yoshikane, Y.; Matsumoto, S.; Fujisawa, M.; Ohnishi, K.; Yagi, T.; J. Biochem. 145, 493-503 (2009) 
Data extracted from this reference:
Engineering
Protein Variants
Commentary
Organism
E149Q
mutant shows a showed a different pH optimum depending on the cosubstrate. With NAD+, the mutant shows very low activity with an optimum pH at 8.5 in the universal buffer. In contrast, the optimum pH is 5.5 with NADH
Mesorhizobium loti
H137L
almost complete loss of activity
Mesorhizobium loti
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0058
-
NAD+
mutant E149Q, pH 8.0, 30°C
Mesorhizobium loti
0.0343
-
NAD+
wild-type, pH 8.0, 30°C
Mesorhizobium loti
0.0428
-
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate
wild-type, pH 8.0, 30°C
Mesorhizobium loti
0.241
-
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate
mutant E149Q, pH 8.0, 30°C
Mesorhizobium loti
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
59100
-
gel filtration
Mesorhizobium loti
Organism
Organism
UniProt
Commentary
Textmining
Mesorhizobium loti
Q988H5
enzyme additionally catalyzes the reaction of 4-pyridoxic acid dehydrogenase
-
Mesorhizobium loti MAFF303099
Q988H5
enzyme additionally catalyzes the reaction of 4-pyridoxic acid dehydrogenase
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O
-
745236
Mesorhizobium loti
3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
-
-
-
?
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O
-
745236
Mesorhizobium loti MAFF303099
3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
-
-
-
?
additional information
enzyme catalyzes practically irreversible oxidation of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NAD+ to 3-hydroxy-2-methyl-pyridine 4, 5-dicarboxylic acid, and practically irreversible reduction of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NADH to 4-pyridoxic acid, i.e. reaction of EC 4-pyridoxic acid dehydrogenase. When the enzyme reaction is started with the combination of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NAD+ or that of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NADH, 3-hydroxy-2-methylpyridine-4,5-dicarboxylate and 4-pyridoxic acid are produced in an almost equimolar ratio throughout the reaction
745236
Mesorhizobium loti
?
-
-
-
-
additional information
enzyme catalyzes practically irreversible oxidation of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NAD+ to 3-hydroxy-2-methyl-pyridine 4, 5-dicarboxylic acid, and practically irreversible reduction of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NADH to 4-pyridoxic acid, i.e. reaction of EC 4-pyridoxic acid dehydrogenase. When the enzyme reaction is started with the combination of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NAD+ or that of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NADH, 3-hydroxy-2-methylpyridine-4,5-dicarboxylate and 4-pyridoxic acid are produced in an almost equimolar ratio throughout the reaction
745236
Mesorhizobium loti MAFF303099
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 38900, SDS-PAGE, 2 * 33060, calculated from sequence, 2 * 33121, TOF-MS
Mesorhizobium loti
Synonyms
Synonyms
Commentary
Organism
mlr6739
-
Mesorhizobium loti
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Mesorhizobium loti
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
25
-
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate
mutant E149Q, pH 8.0, 30°C
Mesorhizobium loti
204
-
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate
wild-type, pH 8.0, 30°C
Mesorhizobium loti
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Mesorhizobium loti
Engineering (protein specific)
Protein Variants
Commentary
Organism
E149Q
mutant shows a showed a different pH optimum depending on the cosubstrate. With NAD+, the mutant shows very low activity with an optimum pH at 8.5 in the universal buffer. In contrast, the optimum pH is 5.5 with NADH
Mesorhizobium loti
H137L
almost complete loss of activity
Mesorhizobium loti
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0058
-
NAD+
mutant E149Q, pH 8.0, 30°C
Mesorhizobium loti
0.0343
-
NAD+
wild-type, pH 8.0, 30°C
Mesorhizobium loti
0.0428
-
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate
wild-type, pH 8.0, 30°C
Mesorhizobium loti
0.241
-
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate
mutant E149Q, pH 8.0, 30°C
Mesorhizobium loti
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
59100
-
gel filtration
Mesorhizobium loti
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O
-
745236
Mesorhizobium loti
3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
-
-
-
?
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O
-
745236
Mesorhizobium loti MAFF303099
3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
-
-
-
?
additional information
enzyme catalyzes practically irreversible oxidation of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NAD+ to 3-hydroxy-2-methyl-pyridine 4, 5-dicarboxylic acid, and practically irreversible reduction of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NADH to 4-pyridoxic acid, i.e. reaction of EC 4-pyridoxic acid dehydrogenase. When the enzyme reaction is started with the combination of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NAD+ or that of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NADH, 3-hydroxy-2-methylpyridine-4,5-dicarboxylate and 4-pyridoxic acid are produced in an almost equimolar ratio throughout the reaction
745236
Mesorhizobium loti
?
-
-
-
-
additional information
enzyme catalyzes practically irreversible oxidation of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NAD+ to 3-hydroxy-2-methyl-pyridine 4, 5-dicarboxylic acid, and practically irreversible reduction of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NADH to 4-pyridoxic acid, i.e. reaction of EC 4-pyridoxic acid dehydrogenase. When the enzyme reaction is started with the combination of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NAD+ or that of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NADH, 3-hydroxy-2-methylpyridine-4,5-dicarboxylate and 4-pyridoxic acid are produced in an almost equimolar ratio throughout the reaction
745236
Mesorhizobium loti MAFF303099
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 38900, SDS-PAGE, 2 * 33060, calculated from sequence, 2 * 33121, TOF-MS
Mesorhizobium loti
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Mesorhizobium loti
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
25
-
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate
mutant E149Q, pH 8.0, 30°C
Mesorhizobium loti
204
-
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate
wild-type, pH 8.0, 30°C
Mesorhizobium loti
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Mesorhizobium loti
General Information
General Information
Commentary
Organism
physiological function
contrary to wild-type, mlr6793-disruptant cells cannot grow on pyridoxine, 4-pyridoxic acid or 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate as sole carbon and nitrogen source
Mesorhizobium loti
General Information (protein specific)
General Information
Commentary
Organism
physiological function
contrary to wild-type, mlr6793-disruptant cells cannot grow on pyridoxine, 4-pyridoxic acid or 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate as sole carbon and nitrogen source
Mesorhizobium loti
Other publictions for EC 1.2.1.100
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744227
Mugo
Crystal structure of 5-formyl ...
Mesorhizobium japonicum
Biochem. Biophys. Res. Commun.
456
35-40
2015
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1
1
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2
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3
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2
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1
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1
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1
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1
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2
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2
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1
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-
745236
Yokochi
Gene identification and chara ...
Mesorhizobium loti, Mesorhizobium loti MAFF303099
J. Biochem.
145
493-503
2009
-
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-
2
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4
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1
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5
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4
1
1
1
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2
1
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2
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4
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1
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4
1
1
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2
1
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1
1
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745254
Lee
Enzymes of vitamin B6 degrada ...
Pseudomonas sp. MA-1
J. Biol. Chem.
261
15106-15111
1986
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1
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1
1
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1
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