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Literature summary for 1.2.1.10 extracted from

  • Fischer, B.; Boutserin, S.; Mazon, H.; Collin, S.; Branlant, G.; Gruez, A.; Talfournier, F.
    Catalytic properties of a bacterial acylating acetaldehyde dehydrogenase: evidence for several active oligomeric states and coenzyme A activation upon binding (2013), Chem. Biol. Interact., 202, 70-77.
    View publication on PubMed
Search for more literature for 1.2.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41 (DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, in the absence of cofactor or in the presence of either 5 mM NAD+ or CoA using 22% PEG 4000, 0.1 M HEPES/NaOH pH 7.5, 0.1 M sodium acetate Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
acetaldehyde competitive inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 CoA at 25°C in 50 mM Tris/HCl buffer, pH 8.0 Escherichia coli
0.25
-
 NAD+ at 25°C in 50 mM Tris/HCl buffer, pH 8.0 Escherichia coli
38
-
 acetaldehyde at 25°C in 50 mM Tris/HCl buffer, pH 8.0 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33344
-
 1 * 33344, electrospray ionization mass spectrometry Escherichia coli
35300
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetaldehyde + CoA + NAD+ Escherichia coli
-
 acetyl-CoA + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + CoA + NAD+
-
 Escherichia coli acetyl-CoA + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33344, electrospray ionization mass spectrometry Escherichia coli

Synonyms

Synonyms Comment Organism
acylating acetaldehyde dehydrogenase
-
 Escherichia coli
MhpF
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.7
-
 acetaldehyde at 25°C in 50 mM Tris/HCl buffer, pH 8.0 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
265
-
 acetaldehyde at 25°C in 50 mM Tris/HCl buffer, pH 8.0 Escherichia coli