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Literature summary for 1.2.1.10 extracted from

  • Kazahaya, T.; Kawai, K.; Yashima, S.; Sasaki, Y.
    Aerobic dissimilation of glucose by heterolactic bacteria. III. Aldehyde dehydrogenase and alcohol dehydrogenase of Leuconostoc mesenteroides (1972), J. Gen. Appl. Microbiol., 18, 43-55.
No PubMed abstract available
Search for more literature for 1.2.1.10

Activating Compound

Activating Compound Comment Organism Structure
GSH 7fold activation Leuconostoc mesenteroides

Inhibitors

Inhibitors Comment Organism Structure
ADP 1 mM 42% inhibition, competitive to NAD+ Leuconostoc mesenteroides
AgNO3 1 mM 20% inhibition Leuconostoc mesenteroides
AMP 1 mM 48% inhibition, competitive to NAD+ Leuconostoc mesenteroides
ATP 1 mM 38% inhibition, competitive to NAD+ Leuconostoc mesenteroides
CuSO4 1 mM 49% inhibition Leuconostoc mesenteroides
HgCl2 1 mM 31% inhibition Leuconostoc mesenteroides
additional information low ionic strength buffers, 0.2 M boric acid/Na2CO3, 0.2 M glycine/NaOH, 0.2 M NH4Cl/NH4OH, pH 8.0, not inhibitory at 1 mM: K+, Na+, Li+, Mg2+, Mn2+, Zn2 +, Ca2+, Fe2+, Fe3+, EDTA, p-chloromercuribenzoate, iodoacetate, N3-, dipyridyl, lactic acid, acetic acid, ethanol, acetylphosphate, pyruvic acid, adenine, adenosine, guanine, guanosine, GMP, GDP, not inhibitory at 0.1 mM: NADP+, NADPH Leuconostoc mesenteroides
Na2HAsO4 1 mM 100% inhibition, 0.1 mM 32% inhibition Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 CoA
-
 Leuconostoc mesenteroides
0.17
-
 NAD+
-
 Leuconostoc mesenteroides
3.7
-
 acetaldehyde
-
 Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetaldehyde + CoA + NAD+ Leuconostoc mesenteroides ethanol production in aerobic glucose dissimilation acetyl-CoA + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
 IFO 3426
-

Purification (Commentary)

Purification (Comment) Organism
partial Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.87
-
-
 Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + CoA + NAD+
-
 Leuconostoc mesenteroides acetyl-CoA + NADH
-
 r
acetaldehyde + CoA + NAD+ ethanol production in aerobic glucose dissimilation Leuconostoc mesenteroides acetyl-CoA + NADH
-
 r
isobutyraldehyde + CoA + NAD+
-
 Leuconostoc mesenteroides isobutyryl-CoA + NADH
-
 ?
additional information not: benzaldehyde Leuconostoc mesenteroides ?
-
 ?
n-butyraldehyde + CoA + NAD+
-
 Leuconostoc mesenteroides n-butyryl-CoA + NADH
-
 r
propanal + CoA + NAD+
-
 Leuconostoc mesenteroides propionyl-CoA + NADH
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Leuconostoc mesenteroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 15 min, 30% activity left Leuconostoc mesenteroides
45
-
 15 min, inactivation Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Leuconostoc mesenteroides

Cofactor

Cofactor Comment Organism Structure
CoA
-
 Leuconostoc mesenteroides
NAD+ no reaction with NADP+ Leuconostoc mesenteroides

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.75
-
 AMP
-
 Leuconostoc mesenteroides
2.5
-
 ADP
-
 Leuconostoc mesenteroides
8.6
-
 ATP
-
 Leuconostoc mesenteroides