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Literature summary for 1.2.1.10 extracted from

  • Hosoi, N.; Ozaki, C.; Kitamoto, Y.; Ichikawa, Y.
    Purification and properties of aldehyde dehydrogenase (acylating) from propionibacterium freudenreichii (1979), J. Ferment. Technol., 57, 418-427.
No PubMed abstract available
Search for more literature for 1.2.1.10

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
 Propionibacterium freudenreichii
sulfhydryl compound requirement for reduced form Propionibacterium freudenreichii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 CoA
-
 Propionibacterium freudenreichii
0.43
-
 NAD+
-
 Propionibacterium freudenreichii
1.5
-
 Valeraldehyde
-
 Propionibacterium freudenreichii
6.9
-
 propanal
-
 Propionibacterium freudenreichii
100
-
 acetaldehyde
-
 Propionibacterium freudenreichii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 4 * 48000, SDS-PAGE Propionibacterium freudenreichii
188000
-
 gel filtration Propionibacterium freudenreichii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetaldehyde + CoA + NAD+ Propionibacterium freudenreichii
-
 acetyl-CoA + NADH
-
 ?
propanal + CoA + NAD+ Propionibacterium freudenreichii fermentation of 1,2-propanediol propionyl-CoA + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Propionibacterium freudenreichii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Propionibacterium freudenreichii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
83.7
-
-
 Propionibacterium freudenreichii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + CoA + NAD+
-
 Propionibacterium freudenreichii acetyl-CoA + NADH
-
 ?
glutaraldehyde + CoA + NAD+
-
 Propionibacterium freudenreichii glutaryl-CoA + NADH
-
 ?
glyoxal + CoA + NAD+
-
 Propionibacterium freudenreichii glyoxyl-CoA + NADH
-
 ?
heptylaldehyde + CoA + NAD+
-
 Propionibacterium freudenreichii heptanoyl-CoA + NADH
-
 ?
hexylaldehyde + CoA + NAD+
-
 Propionibacterium freudenreichii hexanoyl-CoA + NADH
-
 ?
isobutyraldehyde + CoA + NAD+
-
 Propionibacterium freudenreichii isobutyryl-CoA + NADH
-
 ?
additional information not: formaldehyde, octylaldehyde, benzaldehyde, D,L-glyceraldehyde, glycolaldehyde Propionibacterium freudenreichii ?
-
 ?
n-butyraldehyde + CoA + NAD+
-
 Propionibacterium freudenreichii n-butyryl-CoA + NADH
-
 r
propanal + CoA + NAD+ fermentation of 1,2-propanediol Propionibacterium freudenreichii propionyl-CoA + NADH
-
 ?
propanal + CoA + NAD+ fermentation of 1,2-propanediol Propionibacterium freudenreichii propionyl-CoA + NADH
-
 r
valeraldehyde + CoA + NAD+
-
 Propionibacterium freudenreichii valeryl-CoA + NADH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 48000, SDS-PAGE Propionibacterium freudenreichii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Propionibacterium freudenreichii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 55% of maximal activity at 20°C, 60% at 60°C Propionibacterium freudenreichii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 3 min, inactivation above Propionibacterium freudenreichii

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 30% of maximal activity at pH 7.0, 100% at pH 9.5 Propionibacterium freudenreichii
8.2 9.5
-
 Propionibacterium freudenreichii

Cofactor

Cofactor Comment Organism Structure
CoA
-
 Propionibacterium freudenreichii
NAD+ no reaction with NADP+ Propionibacterium freudenreichii