Cloned (Comment) | Organism |
---|---|
gene nifD, expression of wild-type and mutant enzymes | Azotobacter vinelandii |
Protein Variants | Comment | Organism |
---|---|---|
alphaH195Q | site-directed mutagenesis, the mutant shows an altered electron transfer substrate specificity compared to the wild-type enzyme,overview | Azotobacter vinelandii |
alphaQ191K | site-directed mutagenesis, the mutant shows reduced electron transfer activity compared to the wild-type enzyme, it reduces only H+, not N2, overview | Azotobacter vinelandii |
additional information | construction of Qalpha191K, Halpha195Q, nifValpha, Qalpha191K/nifValpha and Halpha195Q/nifValpha mutants and determination of their substrate specificity, the mutant strains all show reduced electron transfer, overview | Azotobacter vinelandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | as part of the Fe protein in the MoFe protein, [8Fe-7S] clusters | Azotobacter vinelandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6 reduced flavodoxin + N2 + 6 H2O + 6 ATP | Azotobacter vinelandii | - |
6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
H+ and N2 reduction activities of wild-type and mutant Azotobacter vinelandii strains under 100% N2, overview | Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6 reduced flavodoxin + N2 + 6 H2O + 6 ATP | - |
Azotobacter vinelandii | 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate | - |
? | |
6 reduced flavodoxin + N2 + 6 H2O + 6 ATP | analysis of active sites for N2 and H+ reduction on FeMo-cofactor of nitrogenase | Azotobacter vinelandii | 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Azotobacter vinelandii | |
flavodoxin | - |
Azotobacter vinelandii | |
MoFe protein | two parallel electron channels may exist between the [8Fe-7S] cluster and FeMo-cofactor | Azotobacter vinelandii |