KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | over the temperature range 30-45 degrees C the apparent Km for the reduction of N2 is the same, but increases from 30 to 58 kPa of N2 | Azotobacter chroococcum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter chroococcum | P15332 and P15334 and P15333 | P15332 i.e. subunit VnfD, P15334 i.e. subunit VnfK, P15333 i.e. subunit VnfG | - |
Azotobacter chroococcum MCD1 | P15332 and P15334 and P15333 | P15332 i.e. subunit VnfD, P15334 i.e. subunit VnfK, P15333 i.e. subunit VnfG | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O | - |
Azotobacter chroococcum | 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate | - |
? | |
12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O | - |
Azotobacter chroococcum MCD1 | 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
vnfD | alpha-chain | Azotobacter chroococcum |
vnfG | delta-chain | Azotobacter chroococcum |
vnfK | beta-chain | Azotobacter chroococcum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 50 | on increasing the temperature from 45 degrees C to 50 degrees C, little change occurrs in the rate of reduction of protons to H2. The rate of N2H4 production increases, but the rate of NH3 formation decreases 7fold. This selective loss of the ability to reduce N2 to NH3 is reversible | Azotobacter chroococcum |