Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 150 mM ACES, 75 mM Tris, 75 mM ethanolamine (pH 9.8), 17-18% (w/v) PEG 3350, 0.8 M NaCl, and 1 mM sodium dithionite | Azotobacter vinelandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains iron | Azotobacter vinelandii | |
Molybdenum | contains molybdenum | Azotobacter vinelandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | P07328 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylene + dithionite + H+ + ATP + H2O | - |
Azotobacter vinelandii | ethylene + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 233000, SDS-PAGE | Azotobacter vinelandii |
Synonyms | Comment | Organism |
---|---|---|
nitrogenase MoFe-protein | - |
Azotobacter vinelandii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Azotobacter vinelandii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
the enzyme is stable when incubated at pH 9.5. At higher pH values and under turnover conditions, the enzyme is slowly inactivated. Initially the enzyme is reversibly inhibited (about 90%) for substrate reduction at pH 9.5, yet in a second, slower process, the enzyme becomes irreversibly inactivated as measured by substrate reduction at the optimal pH of 7.8. Incubation of the enzyme alone at pH 9.5 shows only minimal activity loss even after 4 h | Azotobacter vinelandii |