Protein Variants | Comment | Organism |
---|---|---|
V70A | site-directed mutagenesis, increased the hydrazine reduction activity, reduced Km comapred to the wild-type enzyme | Azotobacter vinelandii |
V70I | site-directed mutagenesis, decreased the hydrazine reduction activity | Azotobacter vinelandii |
V70X | site-directed mutagenesis, substitution of valine with an amino acid with a smaller side chain increases the hydrazine reduction activity, substitution with an amino acid with a larger side chain decreases the enzyme activity with N2, acetylene or hydrazine | Azotobacter vinelandii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N2 | inhibits hydrazine reduction | Azotobacter vinelandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for N2 of wild-type and mutant enzyme | Azotobacter vinelandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Azotobacter vinelandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced ferredoxin + H+ + N2 + ATP + H2O | Azotobacter vinelandii | - |
oxidized ferredoxin + H2 + NH3 + ADP + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | active site location | Azotobacter vinelandii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.13 | - |
V70I mutant, substrate acetylene, in presence of C2H4 | Azotobacter vinelandii |
0.17 | - |
V70I mutant, substrate N2, in presence of NH3 | Azotobacter vinelandii |
0.2 | - |
wild-type enzyme, substrate acetylene, in presence of H2 | Azotobacter vinelandii |
0.6 | - |
wild-type enzyme, substrate N2, in presence of NH3 | Azotobacter vinelandii |
0.64 | - |
wild-type enzyme, substrate N2, in presence of H2 | Azotobacter vinelandii |
1.8 | - |
wild-type enzyme, substrate acetylene, in presence of C2H4 | Azotobacter vinelandii |
1.94 | - |
V70I mutant, substrate N2, in presence of H2 | Azotobacter vinelandii |
2 | - |
V70I mutant, substrate acetylene, in presence of H2 | Azotobacter vinelandii |
2.02 | - |
wild-type enzyme, substrate argon, in presence of H2 | Azotobacter vinelandii |
2.34 | - |
V70I mutant, substrate argon, in presence of H2 | Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylene + ? | - |
Azotobacter vinelandii | ethylene + ? | - |
? | |
hydrazine + ? | high activity with the Val70 mutant enzyme, poor substrate for the wild-type enzyme | Azotobacter vinelandii | ? | - |
? | |
reduced ferredoxin + H+ + N2 + ATP + H2O | - |
Azotobacter vinelandii | oxidized ferredoxin + H2 + NH3 + ADP + phosphate | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Azotobacter vinelandii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | assay at, hydrazine reduction activity | Azotobacter vinelandii |
7 | - |
assay at, N2 reduction activity | Azotobacter vinelandii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Azotobacter vinelandii | |
iron-molybdenum cofactor | substrate interaction at an iron-sulfur face of the FeMo-cofactor during nitrogenase catalysis | Azotobacter vinelandii |