Protein Variants | Comment | Organism |
---|---|---|
H195Q | below 2% N2 reducing activity remaining compared to wild-type due to less effective N2 binding | Azotobacter vinelandii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N2 | inhibits C2H2 reduction of mutant H195Q; maximal inhibition of H2 production at Fe protein to MoFe protein ration 2.5 | Azotobacter vinelandii | |
N3- | inhibits H2 production competitively and reversibly | Azotobacter vinelandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Azotobacter vinelandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ required for MgATP complex | Azotobacter vinelandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Azotobacter vinelandii | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant H195Q | Azotobacter vinelandii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Azotobacter vinelandii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay in anaerobic atmosphere required | Azotobacter vinelandii |
0.024 | - |
purified mutant H195Q enzyme, anaerobic atmosphere | Azotobacter vinelandii |
0.7 | - |
purified enzyme, anaerobic atmosphere | Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Azotobacter vinelandii | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
reduced ferredoxin + H+ + ATP | - |
Azotobacter vinelandii | oxidized ferredoxin + H2 + ADP + phosphate | - |
? | |
reduced ferredoxin + H+ + N3- + ATP | mutant H195Q shows only about 7.5% activity compared to wild-type | Azotobacter vinelandii | oxidized ferredoxin + NH3 + N2 + ADP + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Azotobacter vinelandii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
13 | 45 | - |
Azotobacter vinelandii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Azotobacter vinelandii |