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Literature summary for 1.18.6.1 extracted from

  • Whitting, M.J.; Dilworth, M.J.
    Legume root nodule nitrogenase. Purification, properties, and studies on its genetic control (1974), Biochim. Biophys. Acta, 371, 337-351.
    View publication on PubMed
Search for more literature for 1.18.6.1

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 4 atoms of iron per molecule of Fe protein Bradyrhizobium lupini
Molybdenum
-
 Bradyrhizobium lupini

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
 Bradyrhizobium lupini
65000
-
 Fe protein, gel filtration Bradyrhizobium lupini
194000
-
 MoFe protein, gel filtration Bradyrhizobium lupini

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Bradyrhizobium lupini
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bradyrhizobium lupini
-
-
-

Oxidation Stability

Oxidation Stability Organism
extreme O2 lability, t1/2: 1 min, Fe protein Bradyrhizobium lupini

Purification (Commentary)

Purification (Comment) Organism
both components Bradyrhizobium lupini

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Bradyrhizobium lupini
a

Source Tissue

Source Tissue Comment Organism Textmining
root nodule bacteroid Bradyrhizobium lupini
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.43
-
 purified Fe protein Bradyrhizobium lupini
0.7
-
 purified MoFe protein Bradyrhizobium lupini

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Bradyrhizobium lupini 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?