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Literature summary for 1.18.6.1 extracted from

  • Kavanagh, E.P.; Hill, S.
    Oxygen inhibition of nitrogenase activity in Klebsiella pneumoniae (1992), J. Gen. Microbiol., 139, 1307-1314.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
O2 complete reversible inhibition, reversibility decreases by increasing the time of exposure to O2 Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Klebsiella pneumoniae
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Oxidation Stability

Oxidation Stability Organism
complete reversible inhibition by O2, reversibility decreases by increasing the time of exposure to O2, after 20 min 60% reversibility of the inhibition remains Klebsiella pneumoniae

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.027
-
reductant dithionite, whole cell assay Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Klebsiella pneumoniae 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
dithionite + H+ + N2 + ATP in vitro substrate Klebsiella pneumoniae ?
-
?