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Literature summary for 1.18.1.5 extracted from

  • Sevrioukova, I.F.; Poulos, T.L.
    Putidaredoxin reductase, a new function for an old protein (2002), J. Biol. Chem., 277, 25831-25839.
    View publication on PubMed
Search for more literature for 1.18.1.5

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information expression of wild-type as His-tagged protein. Molecular, spectral, and electron transferring properties of recombinant His6-Pdr to artificial and native electron acceptors are similar to those of the wild-type protein. Contrary to wild-type, under anaerobic conditions, NAD+ induces in His6 Pdr spectral changes indicative of flavin reduction and formation of the charge transfer complex between the reduced FAD and NAD+ Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida P16640
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fe(CN)62- + NAD+
-
 Pseudomonas putida Fe(CN)63- + NADH + H+
-
 ?
additional information wild-type and His6 Pdr are able to function as NAD(H)-dependent dithiol/disulfide oxidoreductases catalyzing both forward and reverse reactions, NAD+-dependent oxidation of thiols, and NADH-dependent reduction of disulfides. This function of the flavoprotein can be dissociated from electron transfer to putidaredoxin Pseudomonas putida ?
-
 ?
reduced 2,6-dichlorophenolindophenol + NAD+
-
 Pseudomonas putida oxidized 2,6-dichlorophenolindophenol + NADH + H+
-
 ?
reduced putidaredoxin + NAD+
-
 Pseudomonas putida oxidized putidaredoxin + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
dimer both WT and His6-Pdr undergo a monomer-dimer association-dissociation Pseudomonas putida
monomer both WT and His6-Pdr undergo a monomer-dimer association-dissociation Pseudomonas putida

Synonyms

Synonyms Comment Organism
Pdr
-
 Pseudomonas putida
putidaredoxin reductase
-
 Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
126
-
 Fe(CN)62- pH 8.0, 25°C Pseudomonas putida
264
-
 reduced putidaredoxin pH 8.0, 25°C Pseudomonas putida
671
-
 Fe(CN)62- pH 8.0, 25°C Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+ both WT and His6-Pdr are partially present as an NAD+-bound form Pseudomonas putida