Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.18.1.4 extracted from

  • Ma, K.; Adams, M.W.W.
    NAD(P)H:rubredoxin oxidoreductase from Pyrococcus furiosus (2001), Methods Enzymol., 334, 55-62.
    View publication on PubMed
Search for more literature for 1.18.1.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 NADPH
-
 Pyrococcus furiosus
0.0095
-
 oxidized rubredoxin 25°C Pyrococcus furiosus
0.034
-
 NADH
-
 Pyrococcus furiosus
0.05
-
 oxidized rubredoxin 80°C Pyrococcus furiosus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Pyrococcus furiosus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxidized rubredoxin + NADPH Pyrococcus furiosus
-
 reduced rubredoxin + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
 DSM 3638
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1000
-
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome c + NADH cytochrome c from horse heart Pyrococcus furiosus 2 ferrocytochrome c + NAD+ + H+
-
 ?
2,6-dichloroindophenol + NADH
-
 Pyrococcus furiosus reduced 2,6-dichloroindophenol + NAD+ + H+
-
 ?
5,5'-dithiobis(2-nitrobenzoic acid) + NADH
-
 Pyrococcus furiosus ?
-
 ?
benzyl viologen + NADH
-
 Pyrococcus furiosus reduced benzyl viologen + NAD+
-
 ?
FAD + NADH
-
 Pyrococcus furiosus FADH2 + NAD+
-
 ?
Fe(III) citrate + NADH
-
 Pyrococcus furiosus Fe(II) citrate + NAD+
-
 ?
FMN + NADH
-
 Pyrococcus furiosus FMNH2 + NAD+
-
 ?
methyl viologen + NADH
-
 Pyrococcus furiosus reduced methyl viologen + NAD+ + H+
-
 ?
oxidized rubredoxin + NADH rubredoxin is the best substrate Pyrococcus furiosus reduced rubredoxin + NAD+
-
 ?
oxidized rubredoxin + NADPH
-
 Pyrococcus furiosus reduced rubredoxin + NADP+
-
 ?
oxidized rubredoxin + NADPH
-
 Pyrococcus furiosus reduced rubredoxin + NADP+ + H+
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 above Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
FAD enzyme contains FAD Pyrococcus furiosus
FMN
-
 Pyrococcus furiosus
NADH
-
 Pyrococcus furiosus
NADPH preferred physiological electron donor Pyrococcus furiosus