Literature summary for 1.18.1.3 extracted from

Subramanian, V.; Liu, T.N.; Yeh, W.K.; Narro, M.; Gibson, D.T.
Purification and properties of NADH-ferredoxinTOL reductase. A component of toluene dioxygenase from Pseudomonas putida (1981), J. Biol. Chem., 256, 2723-2730.

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Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	-	Pseudomonas putida
2,2'-dipyridyl	-	Pseudomonas putida
PCMB	-	Pseudomonas putida

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0046	-	ferricytochrome c	-	Pseudomonas putida
0.0105	-	NADH	reaction with cytochrome c	Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46000	-	1 * 46000, SDS-PAGE	Pseudomonas putida
46500	-	gel filtration	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	NAD-ferredocinTOL reductase component of toluene dioxygenase	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
12.3	-	-	Pseudomonas putida

Storage Stability

Storage Stability	Organism
-20°C, 3 months, little loss of activity	Pseudomonas putida
0-4°C, stable for up to 30 h	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 ferricyanide + NADH	-	Pseudomonas putida	2 ferrocyanide + NAD+ + H+	-	?
2 ferricytochrome c + NADH	-	Pseudomonas putida	2 ferrocytochrome c + NAD+ + H+	-	?
ferricytochrome c + NADH	-	Pseudomonas putida	ferrocytochrome c + NAD+ + H+	-	r
NADH + oxidized 2,6-dichlorophenolindophenol + H+	-	Pseudomonas putida	NAD+ + reduced 2,6-dichlorophenolindophenol	-	?
NADH + oxidized nitro blue tetrazolium + H+	-	Pseudomonas putida	NAD+ + reduced nitro blue tetrazolium	-	?
oxidized ferredoxin + NADH	-	Pseudomonas putida	reduced ferredoxin + NAD+	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 46000, SDS-PAGE	Pseudomonas putida

Synonyms

Synonyms	Comment	Organism
NAD-ferredocinTOL reductase	component of toluene dioxygenase	Pseudomonas putida

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.6	7.5	-	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
FAD	can bind one mol of FAD per mol of enzyme, Km: 2.5 nM	Pseudomonas putida
NADH	-	Pseudomonas putida

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Release 2023.1 (January 2023)