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Literature summary for 1.18.1.2 extracted from

  • Seo, D.; Asano, T.; Komori, H.; Sakurai, T.
    Role of the C-terminal extension stacked on the re-face of the isoalloxazine ring moiety of the flavin adenine dinucleotide prosthetic group in ferredoxin-NADP+ oxidoreductase from Bacillus subtilis (2014), Plant Physiol. Biochem., 81, 143-148 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tuner(DE3) cells Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information the depletions of residues Y313 to K332 (whole C-terminal extension region) and S325 to K332 results in significant increases in the catalytic efficiency with NADPH in diaphorase assay with ferricyanide, whereas Km values for ferricyanide are increased. In the cytochrome c reduction assay in the presence of ferredoxin, the S325-K332 depleted mutant displays a significant decrease in the turnover rate. The Y313-K332 depleted mutant demonstrates an increase in the rate of the direct reduction of horse heart cytochrome c in the absence of ferredoxin Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
NADPH with ferricyanide as cosubstrate, at pH 7.0 and 25°C Bacillus subtilis
0.29
-
ferricyanide at pH 7.0 and 25°C Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
reduced ferredoxin + NADP+ + H+ Bacillus subtilis
-
oxidized ferredoxin + NADPH
-
r
reduced ferredoxin + NADP+ + H+ Bacillus subtilis 168
-
oxidized ferredoxin + NADPH
-
r

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis 168
-
-
-

Purification (Commentary)

Purification (Comment) Organism
hydroxyapatite column chromatography and Mono Q column chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ferricyanide + NADPH
-
Bacillus subtilis ferrocyanide + NADP+ + H+
-
r
ferricyanide + NADPH
-
Bacillus subtilis 168 ferrocyanide + NADP+ + H+
-
r
reduced ferredoxin + NADP+ + H+
-
Bacillus subtilis oxidized ferredoxin + NADPH
-
r
reduced ferredoxin + NADP+ + H+
-
Bacillus subtilis 168 oxidized ferredoxin + NADPH
-
r

Subunits

Subunits Comment Organism
homodimer 2 * 40000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
ferredoxin-NADP+ oxidoreductase
-
Bacillus subtilis
FNR
-
Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
930
-
NADPH with ferricyanide as cosubstrate, at pH 7.0 and 25°C Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
FAD
-
Bacillus subtilis
NADP+
-
Bacillus subtilis