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Literature summary for 1.18.1.2 extracted from

  • Sato, J.; Takeda, K.; Nishiyama, R.; Watanabe, T.; Abo, M.; Yoshimura, E.; Nakagawa, J.; Abe, A.; Kawasaki, S.; Niimura, Y.
    Synechocystis ferredoxin-NADP+ oxidoreductase is capable of functioning as ferric reductase and of driving the Fenton reaction in the absence or presence of free flavin (2011), Biometals, 24, 311-321.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
2 * 34000, SDS-PAGE Synechocystis sp.
60000
-
gel filtration Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp. Q55318
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Synechocystis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.31
-
substrate Fe(III)-EDTA, pH 7.0, 25°C Synechocystis sp.
0.39
-
substrate ferricytochrome c, pH 7.0, 25°C Synechocystis sp.
5.92
-
substrate FMN, pH 7.0, 25°C Synechocystis sp.
6
-
substrate FAD, pH 7.0, 25°C Synechocystis sp.
35.27
-
substrate 2,6-dichlorophenolindophenol, pH 7.0, 25°C Synechocystis sp.
35.28
-
substrate ferricyanide, pH 7.0, 25°C Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADPH
-
Synechocystis sp. 2 ferrocyanide + NADP+ + H+
-
?
2 ferricytochrome c2 + NADPH
-
Synechocystis sp. 2 ferrocytochrome c2 + NADP+ + H+
-
?
FAD + NADPH + H+
-
Synechocystis sp. reduced FAD + NADP+
-
?
Fe(III)-EDTA + NADPH + H+
-
Synechocystis sp. Fe(II) + EDTA + NADP+
-
?
FMN + NADPH + H+
-
Synechocystis sp. reduced FMN + NADP+
-
?
additional information DNA degradation occurring in the presence of NADPH, Fe(III)-EDTA and hydrogen peroxide is potently enhanced by the purified enzyme. The enzyme is capable of functioning as ferric reductase and of driving the Fenton reaction in the absence or presence of free flavin Synechocystis sp. ?
-
?
oxidized 2,6-dichlorophenolindophenol + NADPH + H+
-
Synechocystis sp. reduced 2,6-dichlorophenolindophenol + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE Synechocystis sp.

Synonyms

Synonyms Comment Organism
FNRS
-
Synechocystis sp.
PETH
-
Synechocystis sp.

Cofactor

Cofactor Comment Organism Structure
FAD and FMN, ratio of FAD to FMN is 7.5 to 1 Synechocystis sp.
FMN and FAD, ratio of FAD to FMN is 7.5 to 1 Synechocystis sp.