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Literature summary for 1.18.1.2 extracted from

  • Ikeda, T.; Nakamura, M.; Arai, H.; Ishii, M.; Igarashi, Y.
    Ferredoxin-NADP reductase from the thermophilic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6 (2009), FEMS Microbiol. Lett., 297, 124-130.
    View publication on PubMed
Search for more literature for 1.18.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as recombinant protein Hydrogenobacter thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Fpr showed high specificity for NADPH; Km value for NADPH is <5 microMol, whereas that for NADH is above 2 mM Hydrogenobacter thermophilus
0.131
-
 NADP+
-
 Hydrogenobacter thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24600
-
 gel filtration Hydrogenobacter thermophilus
30000
-
 SDS-PAGE Hydrogenobacter thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxidized ferredoxin + NADPH Hydrogenobacter thermophilus Hydrogenobacter thermophilus expresses three ferredoxins: [4Fe-4S]-type Fd1 and Fd2, and [2Fe-2S]-type Fd3 reduced ferredoxin + NADP+
-
 r
oxidized ferredoxin + NADPH Hydrogenobacter thermophilus TK-6 / IAM 12695 Hydrogenobacter thermophilus expresses three ferredoxins: [4Fe-4S]-type Fd1 and Fd2, and [2Fe-2S]-type Fd3 reduced ferredoxin + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Hydrogenobacter thermophilus
-
-
-
Hydrogenobacter thermophilus TK-6 / IAM 12695
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hydrogenobacter thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.2
-
 Vmax value is 28 micromol/min/mg when NADPH is used as the electron donor, Vmax value is 22 micromol/min/mg when reduced ferredoxin Fd1 is used as the electron donor and NADP+ as electron acceptor Hydrogenobacter thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Hydrogenobacter thermophilus NADP+ + reduced 2,6-dichlorophenolindophenol
-
 r
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Hydrogenobacter thermophilus TK-6 / IAM 12695 NADP+ + reduced 2,6-dichlorophenolindophenol
-
 r
oxidized ferredoxin + NADPH
-
 Hydrogenobacter thermophilus reduced ferredoxin + NADP+
-
 r
oxidized ferredoxin + NADPH Hydrogenobacter thermophilus expresses three ferredoxins: [4Fe-4S]-type Fd1 and Fd2, and [2Fe-2S]-type Fd3 Hydrogenobacter thermophilus reduced ferredoxin + NADP+
-
 r
oxidized ferredoxin + NADPH
-
 Hydrogenobacter thermophilus TK-6 / IAM 12695 reduced ferredoxin + NADP+
-
 r
oxidized ferredoxin + NADPH Hydrogenobacter thermophilus expresses three ferredoxins: [4Fe-4S]-type Fd1 and Fd2, and [2Fe-2S]-type Fd3 Hydrogenobacter thermophilus TK-6 / IAM 12695 reduced ferredoxin + NADP+
-
 r
reduced ferredoxin + NADP+ reaction is performed in presence of CoA Hydrogenobacter thermophilus oxidized ferredoxin + NADPH
-
 r
reduced ferredoxin + NADP+ reaction is performed in presence of CoA Hydrogenobacter thermophilus TK-6 / IAM 12695 oxidized ferredoxin + NADPH
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 24600, gel filtration Hydrogenobacter thermophilus

Synonyms

Synonyms Comment Organism
ferredoxin-NADP+ reductase
-
 Hydrogenobacter thermophilus
FNR
-
 Hydrogenobacter thermophilus
FPR
-
 Hydrogenobacter thermophilus

Cofactor

Cofactor Comment Organism Structure
FMN protein contains flavin mononucleotide instead of FAD Hydrogenobacter thermophilus