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Literature summary for 1.18.1.2 extracted from

  • Goni, G.; Serrano, A.; Frago, S.; Hervas, M.; Peregrina, J.R.; De la Rosa, M.A.; Gomez-Moreno, C.; Navarro, J.A.; Medina, M.
    Flavodoxin-mediated electron transfer from photosystem I to ferredoxin-NADP+ reductase in Anabaena: role of flavodoxin hydrophobic residues in protein-protein interactions (2008), Biochemistry, 47, 1207-1217.
    View publication on PubMed
Search for more literature for 1.18.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Anabaena sp.

Protein Variants

Protein Variants Comment Organism
I59A kcat for flavodoxin is slightly decreased, but kcat/Km is markedly increased Anabaena sp.
I59A/I92A kcat and kcat/Km for flavodoxin are markedly reduced Anabaena sp.
I59E kcat for flavodoxin is increased, kcat/Km is markedly increased Anabaena sp.
I59E/I92E kcat and kcat/Km for flavodoxin are markedly reduced Anabaena sp.
I92A kcat for flavodoxin is increased, kcat/Km is markedly increased Anabaena sp.
I92E kcat for flavodoxin is increased, kcat/Km is markedly increased Anabaena sp.
W57E kcat and kcat/Km for flavodoxin are markedly reduced Anabaena sp.
W57K kcat and kcat/Km for flavodoxin are markedly increased Anabaena sp.
W57R kcat for flavodoxin is increased Anabaena sp.

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Anabaena sp.
-
 PCC 7119
-

Purification (Commentary)

Purification (Comment) Organism
-
 Anabaena sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced flavodoxin + NADP+
-
 Anabaena sp. oxidized flavodoxin + NADPH + H+
-
 r
reduced flavodoxin mutant I59A + NADP+
-
 Anabaena sp. oxidized flavodoxin mutant I59A + NADPH + H+
-
 r
reduced flavodoxin mutant I59A/I92A + NADP+
-
 Anabaena sp. oxidized flavodoxin mutant I59A/I92A + NADPH + H+
-
 r
reduced flavodoxin mutant I59E + NADP+
-
 Anabaena sp. oxidized flavodoxin mutant I59E + NADPH + H+
-
 r
reduced flavodoxin mutant I59E/I92E + NADP+
-
 Anabaena sp. oxidized flavodoxin mutant I59E/I92E + NADPH + H+
-
 r
reduced flavodoxin mutant I92A + NADP+
-
 Anabaena sp. oxidized flavodoxin mutant I92A + NADPH + H+
-
 r
reduced flavodoxin mutant I92E + NADP+
-
 Anabaena sp. oxidized flavodoxin mutant I92E + NADPH + H+
-
 r
reduced flavodoxin mutant W57E + NADP+
-
 Anabaena sp. oxidized flavodoxin mutant W57E + NADPH + H+
-
 r
reduced flavodoxin mutant W57K + NADP+
-
 Anabaena sp. oxidized flavodoxin mutant W57K + NADPH + H+
-
 r
reduced flavodoxin mutant W57R + NADP+
-
 Anabaena sp. oxidized flavodoxin mutant W57R + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
ferredoxin-NADP+ reductase
-
 Anabaena sp.
FNR
-
 Anabaena sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.8
-
 reduced flavodoxin mutant W57E using cytochrome c as electron acceptor, for mutant W57E Anabaena sp.
14
-
 reduced flavodoxin mutant I59E/I92E using cytochrome c as electron acceptor, for mutant I59E/I92E Anabaena sp.
15.3
-
 reduced flavodoxin mutant I59A/I92A using cytochrome c as electron acceptor, for mutant I59A/I92A Anabaena sp.
19
-
 reduced flavodoxin mutant I59A using cytochrome c as electron acceptor, for mutant I59A Anabaena sp.
23.3
-
 reduced flavodoxin using cytochrome c as electron acceptor Anabaena sp.
25.8
-
 reduced flavodoxin mutant I92A using cytochrome c as electron acceptor, for mutant I92A Anabaena sp.
26.5
-
 reduced flavodoxin mutant I92E using cytochrome c as electron acceptor, for mutant I92E Anabaena sp.
27.5
-
 reduced flavodoxin mutant I59E using cytochrome c as electron acceptor, for mutant I59E Anabaena sp.
52.7
-
 reduced flavodoxin mutant W57R using cytochrome c as electron acceptor, for mutant W57R Anabaena sp.
90.3
-
 reduced flavodoxin mutant W57K using cytochrome c as electron acceptor, for mutant W57K Anabaena sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
200
-
 flavodoxin using cytochrome c as electron acceptor, for mutant W57E Anabaena sp.
350
-
 flavodoxin using cytochrome c as electron acceptor, for mutant I59E/I92E Anabaena sp.
450
-
 flavodoxin using cytochrome c as electron acceptor, for mutant I59A/I92A Anabaena sp.
690
-
 flavodoxin using cytochrome c as electron acceptor, for mutant W57R Anabaena sp.
700
-
 flavodoxin using cytochrome c as electron acceptor Anabaena sp.
1010
-
 flavodoxin using cytochrome c as electron acceptor, for mutant W57K Anabaena sp.
2380
-
 flavodoxin using cytochrome c as electron acceptor, for mutant I59A Anabaena sp.
2770
-
 flavodoxin using cytochrome c as electron acceptor, for mutant I92E Anabaena sp.
3090
-
 flavodoxin using cytochrome c as electron acceptor, for mutant I59E Anabaena sp.
3150
-
 flavodoxin using cytochrome c as electron acceptor, for mutant I92A Anabaena sp.