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Literature summary for 1.18.1.2 extracted from

  • Milani, M.; Balconi, E.; Aliverti, A.; Mastrangelo, E.; Seeber, F.; Bolognesi, M.; Zanetti, G.
    Ferredoxin-NADP(+) reductase from Plasmodium falciparum undergoes NADP(+)-dependent dimerization and inactivation: functional and crystallographic analysis (2007), J. Mol. Biol., 367, 501-513.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment) Organism
free enzyme and in complex with 2’-phospho-AMP. Structures reveal a covalent dimer, which relies on the oxidation of residue C99 in two opposing subunits, and a helix-coil transition that occurs in the NADP-binding domain, triggered by 2’-phospho-AMP binding. Studies in solution show that NADP+, as well as 2’-phospho-AMP, promotes the formation of the disulfide-stabilized dimer. The dimer is inactive, but full activity is recovered upon disulfide reduction Plasmodium falciparum
in free form and in complex with 2'-phospho-AMP, in 22-25% PEG 4000, 0.1 M sodium cacodylate (pH 6.0), 0.2 M sodium acetate Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
ferricyanide
-
 Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
 NADPH 25°C, pH 8.2 Plasmodium falciparum
0.036
-
 NADPH with K3Fe(CN)6 as cosubstrate, at 25°C, in 100 mM Tris-HCl (pH 8.2) Plasmodium falciparum
0.095
-
 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride at 25°C in 200 mM Tris-HCl (pH 9.0), containing 70 mM NaCl and 0.1% Triton-X100 Plasmodium falciparum
0.35
-
 NADPH cosubstrate 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride, 25°C, pH 9.0 Plasmodium falciparum
0.35
-
 NADPH with 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride as cosubstrate, at 25°C, in 100 mM Tris-HCl (pH 8.2) Plasmodium falciparum
0.72
-
 NADH 25°C, pH 8.2 Plasmodium falciparum
0.72
-
 NADH with K3Fe(CN)6 as cosubstrate, at 25°C, in 100 mM Tris-HCl (pH 8.2) Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
apicoplast
-
 Plasmodium falciparum 20011
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 gel filtration Plasmodium falciparum
37270
-
 calculated from amino acid sequence Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum C6KT68
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADH
-
 Plasmodium falciparum 2 ferrocyanide + NAD+ + H+
-
 ?
2 ferricyanide + NADPH
-
 Plasmodium falciparum 2 ferrocyanide + NADP+ + H+
-
 ?
2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride + NADPH
-
 Plasmodium falciparum ? + NADP+
-
 ?
2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride + NADPH
-
 Plasmodium falciparum ?
-
 ?
K3Fe(CN)6 + NAD(P)H
-
 Plasmodium falciparum ?
-
 ?
reduced ferredoxin + NADP+
-
 Plasmodium falciparum oxidized ferredoxin + NADPH
-
 r

Subunits

Subunits Comment Organism
dimer the enzyme undergoes NADP+-dependent dimerization (inactive enzyme form) Plasmodium falciparum
monomer 1 * 28000, gel filtration Plasmodium falciparum

Synonyms

Synonyms Comment Organism
Ferredoxin-NADP(+) reductase
-
 Plasmodium falciparum
FNR
-
 Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.185
-
 ferricyanide 25°C, pH 8.2, cosubstrate NADH Plasmodium falciparum
0.23
-
 ferricyanide 25°C, pH 8.2, cosubstrate NADPH Plasmodium falciparum