Protein Variants | Comment | Organism |
---|---|---|
DELTA1-15 | removal of the N-terminal 15 residues of enzyme enhances the interaction with ferredoxin | Toxoplasma gondii |
DELTA81-118 | deleted amino acid comprise a species-specific subdomain containing an insertion of 28 residues, deletion results in an catalytically active enzyme form with 10fold decreased affinity for ferredoxin | Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
reduced ferredoxin | mutant lacking amino acids 81 to 118, pH 8.2, 25°C | Toxoplasma gondii | |
0.015 | - |
NADPH | wild-type, pH 9.0, 25°C | Toxoplasma gondii | |
0.016 | - |
NADPH | mutant lacking amino acids 81 to 118, pH 9.0, 25°C | Toxoplasma gondii | |
0.018 | - |
reduced ferredoxin | wild-type, pH 8.2, 25°C | Toxoplasma gondii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 39000, wild-type, calculated, 1 * 35000, mutant lacking amino acids 81 to 118 | Toxoplasma gondii |
37600 | - |
gel filtration, mutant lacking amino acids 81 to 118 | Toxoplasma gondii |
39000 | - |
1 * 39000, wild-type, calculated, 1 * 35000, mutant lacking amino acids 81 to 118 | Toxoplasma gondii |
46300 | - |
gel filtration, wildtype | Toxoplasma gondii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxidized ferredoxin + NADPH | - |
Toxoplasma gondii | reduced ferredoxin + NADP+ | - |
? | |
reduced ferredoxin + NADP+ | - |
Toxoplasma gondii | oxidized ferredoxin + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 39000, wild-type, calculated, 1 * 35000, mutant lacking amino acids 81 to 118 | Toxoplasma gondii |
More | study on enzyme conformation by limited proteolysis. Protein substrate ferredoxin protects ferredoxin-NADP+ reductase against cleavage both at its N-terminal peptide and at its largest sequence insertion of 28 residues | Toxoplasma gondii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
130 | - |
reduced ferredoxin | mutant lacking pH 8.2, 25°C | Toxoplasma gondii | |
260 | - |
reduced ferredoxin | wild-type, pH 8.2, 25°C | Toxoplasma gondii | |
424 | - |
NADPH | mutant lacking pH 9.0, 25°C | Toxoplasma gondii | |
540 | - |
NADPH | wild-type, pH 9.0, 25°C | Toxoplasma gondii |