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Literature summary for 1.18.1.2 extracted from
- Purification and characterization of a ferredoxin-NADP+ oxidoreductase-like enzyme from radish root tissues (1990), Plant Physiol., 93, 896-901.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| MgCl2 | inhibits at lower salt concentrations | Raphanus sativus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
reduced ferredoxin | - |
Raphanus sativus | |
| 0.0092 | - |
NADPH | - |
Raphanus sativus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | 35000 | gel filtration, SDS-PAGE | Raphanus sativus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Raphanus sativus | - |
radish, var acanthiformis cultivar miyashige | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Raphanus sativus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADPH + oxidized ferredoxin | - |
Raphanus sativus | NADP+ + reduced ferredoxin | - |
? | |
| reduced ferredoxin + NADP+ | - |
Raphanus sativus | oxidized ferredoxin + NADPH + H+ | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
100 mM Tris-HCl | Raphanus sativus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | flavoprotein | Raphanus sativus | |
| additional information | no activity with NADH | Raphanus sativus | |
| NADP+ | requirement for NADPH | Raphanus sativus | |
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