Any feedback?
Literature summary for 1.18.1.1 extracted from
- Rubredoxin reductase from Alcanivorax borkumensis: expression and characterization (2011), Biotechnol. Prog., 27, 1383-1389.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Alcanivorax borkumensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
NADH | pH 8.0, 45°C | Alcanivorax borkumensis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
x * 41000, calculated and SDS-PAGE | Alcanivorax borkumensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alcanivorax borkumensis | Q0VTB0 | - |
- |
| Alcanivorax borkumensis SK2 | Q0VTB0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Alcanivorax borkumensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11500 | - |
pH 8.0, 45°C | Alcanivorax borkumensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + H+ + oxidized methyl thiazolyl tetrazolium | - |
Alcanivorax borkumensis | NAD+ + reduced methyl thiazolyl tetrazolium | - |
? | |
| NADH + H+ + oxidized methyl thiazolyl tetrazolium | - |
Alcanivorax borkumensis SK2 | NAD+ + reduced methyl thiazolyl tetrazolium | - |
? | |
| NADH + H+ + oxidized rubredoxin-2 | i.e. AlkG. Isoform RubB can reduce AlkG, therefore compensating for the absence of AlkT, also a rubredoxin reductase, missing inAlcanivorax borkumensis SK2 genome | Alcanivorax borkumensis | NAD+ + reduced rubredoxin | - |
? | |
| NADH + H+ + oxidized rubredoxin-2 | i.e. AlkG. Isoform RubB can reduce AlkG, therefore compensating for the absence of AlkT, also a rubredoxin reductase, missing inAlcanivorax borkumensis SK2 genome | Alcanivorax borkumensis SK2 | NAD+ + reduced rubredoxin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41000, calculated and SDS-PAGE | Alcanivorax borkumensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ABO_0162 | locus name | Alcanivorax borkumensis |
| rubB | - |
Alcanivorax borkumensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Alcanivorax borkumensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 65 | - |
Alcanivorax borkumensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Alcanivorax borkumensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 12 | - |
Alcanivorax borkumensis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
