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Literature summary for 1.17.99.2 extracted from

  • Johnson, H.A.; Pelletier, D.A.; Spormann, A.M.
    Isolation and characterisation of anaerobic ethylbenzene dehydrogenase, a novel Mo-Fe-S enzyme (2001), J. Bacteriol., 183, 4536-4542.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.99.2

Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli DH5alpha Azoarcus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Azoarcus sp. 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
iron sulfur cluster located in beta-subunit Azoarcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23061
-
 alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.
25000
-
 alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.
35000
-
 alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.
39643
-
 alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.
70000
-
 gel filtration of purified enzyme Azoarcus sp.
100000
-
 alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.
104226
-
 alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.
160000
-
 calculated from DNA-sequence Azoarcus sp.
545000
-
 native PAGE Azoarcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethylbenzene + H2O + acceptor Azoarcus sp. anaerobic degradation of ethylbenzene in bacteria (S)-1-phenylethanol + reduced acceptor
-
 ?
ethylbenzene + H2O + acceptor Azoarcus sp. EB1 anaerobic degradation of ethylbenzene in bacteria (S)-1-phenylethanol + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Azoarcus sp.
-
 EB1
-
Azoarcus sp. EB1
-
 EB1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Azoarcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyl-2-pentene + molybdopterin
-
 Azoarcus sp. ?
-
 ?
3-methyl-2-pentene + molybdopterin
-
 Azoarcus sp. EB1 ?
-
 ?
4-fluoro-ethylbenzene + molybdopterin
-
 Azoarcus sp. ?
-
 ?
4-fluoro-ethylbenzene + molybdopterin
-
 Azoarcus sp. EB1 ?
-
 ?
ethylbenzene + H2O + acceptor anaerobic degradation of ethylbenzene in bacteria Azoarcus sp. (S)-1-phenylethanol + reduced acceptor
-
 ?
ethylbenzene + H2O + acceptor anaerobic degradation of ethylbenzene in bacteria Azoarcus sp. EB1 (S)-1-phenylethanol + reduced acceptor
-
 ?
ethylbenzene + H2O + molybdopterin
-
 Azoarcus sp. (S)-1-phenylethanol + molybdopterin (reduced form)
-
 ?
ethylbenzene + H2O + molybdopterin
-
 Azoarcus sp. EB1 (S)-1-phenylethanol + molybdopterin (reduced form)
-
 ?
ethylidenecyclohexane + molybdopterin
-
 Azoarcus sp. ?
-
 ?
ethylidenecyclohexane + molybdopterin
-
 Azoarcus sp. EB1 ?
-
 ?

Subunits

Subunits Comment Organism
trimer alpha,beta,gamma 1 * 100000 + 1 * 35000 + 1 * 25000, SDS-PAGE, 1 * 104226 + 1 * 39643 + 1 * 23061, calculated from DNA-sequence Azoarcus sp.

Cofactor

Cofactor Comment Organism Structure
molybdopterin
-
 Azoarcus sp.