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Literature summary for 1.17.99.2 extracted from

  • Kniemeyer, O.; Heider, J.
    Ethylbenzene dehydrogenase, a novel hydrocarbon-oxidising molybdenum/iron-sulfur/heme enzyme (2001), J. Biol. Chem., 276, 21381-21386.
    View publication on PubMed
Search for more literature for 1.17.99.2

Inhibitors

Inhibitors Comment Organism Structure
O2 does not affect enzyme in cell extracts, inhibitory for purified enzyme Azoarcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 ethylbenzene
-
 Azoarcus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Azoarcus sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
[Fe(III) (C5H5)2]+
-
 Azoarcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 alpha,beta,gamma 1 * 96000 + 1 * 43000 + 1 * 23000, SDS-PAGE Azoarcus sp.
43000
-
 alpha,beta,gamma 1 * 96000 + 1 * 43000 + 1 * 23000, SDS-PAGE Azoarcus sp.
96000
-
 alpha,beta,gamma 1 * 96000 + 1 * 43000 + 1 * 23000, SDS-PAGE Azoarcus sp.
155000
-
 gel filtration, native PAGE Azoarcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethylbenzene + H2O + acceptor Azoarcus sp. anaerobic degradation of ethylbenzene in bacteria (S)-1-phenylethanol + reduced acceptor
-
 ir
ethylbenzene + H2O + acceptor Azoarcus sp. EbN1 anaerobic degradation of ethylbenzene in bacteria (S)-1-phenylethanol + reduced acceptor
-
 ir

Organism

Organism UniProt Comment Textmining
Azoarcus sp.
-
 EbN1
-
Azoarcus sp. EbN1
-
 EbN1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Azoarcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0035
-
 activity towards n-propylbenzene of cell extract from cells grown on ethylbenzene Azoarcus sp.
0.0224
-
 activity towards ethylbenzene of cell extract from cells grown on ethylbenzene Azoarcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethylbenzene + H2O + acceptor anaerobic degradation of ethylbenzene in bacteria Azoarcus sp. (S)-1-phenylethanol + reduced acceptor
-
 ir
ethylbenzene + H2O + acceptor anaerobic degradation of ethylbenzene in bacteria Azoarcus sp. EbN1 (S)-1-phenylethanol + reduced acceptor
-
 ir
ethylbenzene + H2O + [Fe(III) (C5H5)2]+
-
 Azoarcus sp. (S)-1-phenylethanol + [Fe(II) (C5H5)2]
-
 ir
ethylbenzene + H2O + [Fe(III) (C5H5)2]+
-
 Azoarcus sp. EbN1 (S)-1-phenylethanol + [Fe(II) (C5H5)2]
-
 ir
n-propylbenzene + H2O + [Fe(III) (C5H5)2]+
-
 Azoarcus sp. (S)-1-phenylpropanol + [Fe(II) (C5H5)2]
-
 ?
n-propylbenzene + H2O + [Fe(III) (C5H5)2]+
-
 Azoarcus sp. EbN1 (S)-1-phenylpropanol + [Fe(II) (C5H5)2]
-
 ?

Subunits

Subunits Comment Organism
trimer alpha,beta,gamma 1 * 96000 + 1 * 43000 + 1 * 23000, SDS-PAGE Azoarcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Azoarcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 about maximum activity at both pH Azoarcus sp.