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Literature summary for 1.17.98.4 extracted from
- Selenium-dependent and selenium-independent formate dehydrogenases of Methanococcus vannielii. Separation of the two forms and characterization of the purified selenium-independent form (1981), J. Biol. Chem., 256, 656-663.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Methanococcus vannielii |  |
| 1,2-dihydroxybenzene-3,5-disulfonic acid | - |
Methanococcus vannielii | |
| 2,2'-dipyridyl | - |
Methanococcus vannielii | |
| 4,5-dihydroxybenzene-1,3-disulfonate | - |
Methanococcus vannielii | |
| EDTA | - |
Methanococcus vannielii | |
| iodoacetamide | addition of 1 mM iodoacetamide, to enzyme preparations in pH 7 buffer containing 2 mM 2-mercaptoethanol inactivates | Methanococcus vannielii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| selenium | anaerobic oxidation of formate by Methanococcus vannielii is catalyzed by two readily separable formate dehydrogenases. One of these is a 105000 Da protein that contains molybdenum, iron, and acid-labile sulfide, but not selenium. The other is a high molecular weight complex composed of selenoprotein and molybdo-iron sulfur protein subunits | Methanococcus vannielii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 105000 | - |
anaerobic oxidation of formate by Methanococcus vannielii is catalyzed by two readily separable formate dehydrogenases. One of these is a 105000 Da protein that contains molybdenum, iron, and acid-labile sulfide, but not selenium. The other is a high molecular weight complex composed of selenoprotein and molybdo-iron sulfur protein subunits | Methanococcus vannielii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formate + oxidized coenzyme F420 | Methanococcus vannielii | - |
CO2 + reduced coenzyme F420 | - |
? | |
| formate + oxidized coenzyme F420 | Methanococcus vannielii DSM 1224 | - |
CO2 + reduced coenzyme F420 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanococcus vannielii | - |
- |
- |
| Methanococcus vannielii DSM 1224 | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| extremely oxygen-sensitive | Methanococcus vannielii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formate + FAD | - |
Methanococcus vannielii | CO2 + FADH2 | - |
? | |
| formate + FAD | - |
Methanococcus vannielii DSM 1224 | CO2 + FADH2 | - |
? | |
| formate + FMN | - |
Methanococcus vannielii | CO2 + FMNH2 | - |
? | |
| formate + FMN | - |
Methanococcus vannielii DSM 1224 | CO2 + FMNH2 | - |
? | |
| formate + oxidized coenzyme F420 | - |
Methanococcus vannielii | CO2 + reduced coenzyme F420 | - |
? | |
| formate + oxidized coenzyme F420 | - |
Methanococcus vannielii DSM 1224 | CO2 + reduced coenzyme F420 | - |
? | |
| additional information | no activity with NADP+ or NAD+ | Methanococcus vannielii | ? | - |
? | |
| additional information | no activity with NADP+ or NAD+ | Methanococcus vannielii DSM 1224 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Methanococcus vannielii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9.2 | - |
Methanococcus vannielii |
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Release 2023.1 (January 2023)
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