General Stability | Organism |
---|---|
azide protects the enzyme from inactivation by O2 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | 0.3 mM NaN3, about 80% inhibition | Escherichia coli | |
nitrate | 10 mM NaNO3, 50% inhibition | Escherichia coli | |
nitrite | 10 mM NaNO2, 55% inhibition | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | each mole of enzyme contains 3.3 gatoms of iron | Escherichia coli | |
Se | the enzyme contains selenocysteine | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, formate dehydrogenase component, the formate-hydrogen lyase complex of Escherichia coli decomposes formic acid to hydrogen and carbon dioxide under anaerobic conditions in the absence of exogenous electron acceptors. The complex consists of two separable enzymatic activities: a formate dehydrogenase and a hydrogenase, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Oxidation Stability | Organism |
---|---|
azide protects the enzyme from inactivation by O2 | Escherichia coli |
Purification (Comment) | Organism |
---|---|
the formate dehydrogenase component of the formate-hydrogen lyase complex | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
290 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + benzyl viologen | - |
Escherichia coli | CO2 + reduced benzyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, formate dehydrogenase component, the formate-hydrogen lyase complex of Escherichia coli decomposes formic acid to hydrogen and carbon dioxide under anaerobic conditions in the absence of exogenous electron acceptors. The complex consists of two separable enzymatic activities: a formate dehydrogenase and a hydrogenase, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
FDHH | formate dehydrogenase component of the formate-hydrogen lyase | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.7 | pH 6.5: about 40% of maximal activity, pH 9.7: about 40% of maximal activity | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
room temperature, 20 h, about 45% loss of activity | Escherichia coli |
5.3 | 6.4 | room temperature, 20 h, stable | Escherichia coli |
7 | - |
room temperature, 20 h, about 60% loss of activity | Escherichia coli |