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Literature summary for 1.17.98.4 extracted from

  • Axley, M.J.; Grahame, D.A.; Stadtman, T.C.
    Escherichia coli formate-hydrogen lyase. Purification and properties of the selenium-dependent formate dehydrogenase component (1990), J. Biol. Chem., 265, 18213-18218.
    View publication on PubMed

General Stability

General Stability Organism
azide protects the enzyme from inactivation by O2 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
azide 0.3 mM NaN3, about 80% inhibition Escherichia coli
nitrate 10 mM NaNO3, 50% inhibition Escherichia coli
nitrite 10 mM NaNO2, 55% inhibition Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Fe each mole of enzyme contains 3.3 gatoms of iron Escherichia coli
Se the enzyme contains selenocysteine Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
x * 80000, formate dehydrogenase component, the formate-hydrogen lyase complex of Escherichia coli decomposes formic acid to hydrogen and carbon dioxide under anaerobic conditions in the absence of exogenous electron acceptors. The complex consists of two separable enzymatic activities: a formate dehydrogenase and a hydrogenase, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Oxidation Stability

Oxidation Stability Organism
azide protects the enzyme from inactivation by O2 Escherichia coli

Purification (Commentary)

Purification (Comment) Organism
the formate dehydrogenase component of the formate-hydrogen lyase complex Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
290
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + benzyl viologen
-
Escherichia coli CO2 + reduced benzyl viologen
-
?

Subunits

Subunits Comment Organism
? x * 80000, formate dehydrogenase component, the formate-hydrogen lyase complex of Escherichia coli decomposes formic acid to hydrogen and carbon dioxide under anaerobic conditions in the absence of exogenous electron acceptors. The complex consists of two separable enzymatic activities: a formate dehydrogenase and a hydrogenase, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
FDHH formate dehydrogenase component of the formate-hydrogen lyase Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.7 pH 6.5: about 40% of maximal activity, pH 9.7: about 40% of maximal activity Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.8
-
room temperature, 20 h, about 45% loss of activity Escherichia coli
5.3 6.4 room temperature, 20 h, stable Escherichia coli
7
-
room temperature, 20 h, about 60% loss of activity Escherichia coli