Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.98.3 extracted from

  • Schauer, N.L.; Ferry, J.G.
    Composition of the coenzyme F420-dependent formate dehydrogenase from Methanobacterium formicicum (1986), J. Bacteriol., 165, 405-411.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
azide, FAD, glycerol, and 2-mercaptoethanol stabilize during purification Methanobacterium formicicum

Metals/Ions

Metals/Ions Comment Organism Structure
Iron nonheme iron-sulfur flavoprotein, contains 20.24 iron Methanobacterium formicicum
Zn2+ contains 2 Zn2+ Methanobacterium formicicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
1 * 85000 (alpha-subunit) + 1 * 53000 (beta-subunit), SDS-PAGE Methanobacterium formicicum
85000
-
1 * 85000 (alpha-subunit) + 1 * 53000 (beta-subunit), SDS-PAGE Methanobacterium formicicum
176000
-
gel filtration Methanobacterium formicicum

Organism

Organism UniProt Comment Textmining
Methanobacterium formicicum
-
-
-
Methanobacterium formicicum DSM 2639
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanobacterium formicicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36.2
-
substrate: coenzyme F420, pH and temperature not specified in the publication Methanobacterium formicicum
447
-
substrate: methyl viologen, pH and temperature not specified in the publication Methanobacterium formicicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + oxidized coenzyme F420
-
Methanobacterium formicicum CO2 + reduced coenzyme F420
-
?
formate + oxidized coenzyme F420
-
Methanobacterium formicicum DSM 2639 CO2 + reduced coenzyme F420
-
?

Subunits

Subunits Comment Organism
dimer 1 * 85000 (alpha-subunit) + 1 * 53000 (beta-subunit), SDS-PAGE Methanobacterium formicicum

Synonyms

Synonyms Comment Organism
coenzyme F420-dependent formate dehydrogenase
-
Methanobacterium formicicum

Cofactor

Cofactor Comment Organism Structure
FAD nonheme iron-sulfur flavoprotein, contains 1 FAD molecule. Reduction of the enzyme facilitates dissociation of FAD, and the FAD-depleted enzyme is unable to reduce coenzyme F420. Preincubation of the FAD-depleted enzyme with FAD restores coenzyme F420-dependent activity Methanobacterium formicicum
molybdopterin denaturation of the enzyme releases a molybdopterin cofactor, the enzyme contains 1 mol of molybdenum Methanobacterium formicicum