General Stability | Organism |
---|---|
azide, FAD, glycerol, and 2-mercaptoethanol stabilize during purification | Methanobacterium formicicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | nonheme iron-sulfur flavoprotein, contains 20.24 iron | Methanobacterium formicicum | |
Zn2+ | contains 2 Zn2+ | Methanobacterium formicicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
1 * 85000 (alpha-subunit) + 1 * 53000 (beta-subunit), SDS-PAGE | Methanobacterium formicicum |
85000 | - |
1 * 85000 (alpha-subunit) + 1 * 53000 (beta-subunit), SDS-PAGE | Methanobacterium formicicum |
176000 | - |
gel filtration | Methanobacterium formicicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanobacterium formicicum | - |
- |
- |
Methanobacterium formicicum DSM 2639 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanobacterium formicicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36.2 | - |
substrate: coenzyme F420, pH and temperature not specified in the publication | Methanobacterium formicicum |
447 | - |
substrate: methyl viologen, pH and temperature not specified in the publication | Methanobacterium formicicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + oxidized coenzyme F420 | - |
Methanobacterium formicicum | CO2 + reduced coenzyme F420 | - |
? | |
formate + oxidized coenzyme F420 | - |
Methanobacterium formicicum DSM 2639 | CO2 + reduced coenzyme F420 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 85000 (alpha-subunit) + 1 * 53000 (beta-subunit), SDS-PAGE | Methanobacterium formicicum |
Synonyms | Comment | Organism |
---|---|---|
coenzyme F420-dependent formate dehydrogenase | - |
Methanobacterium formicicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | nonheme iron-sulfur flavoprotein, contains 1 FAD molecule. Reduction of the enzyme facilitates dissociation of FAD, and the FAD-depleted enzyme is unable to reduce coenzyme F420. Preincubation of the FAD-depleted enzyme with FAD restores coenzyme F420-dependent activity | Methanobacterium formicicum | |
molybdopterin | denaturation of the enzyme releases a molybdopterin cofactor, the enzyme contains 1 mol of molybdenum | Methanobacterium formicicum |