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Literature summary for 1.17.9.1 extracted from

  • Chen, Y.; Chao, H.; Zhou, N.
    The catabolism of 2,4-xylenol and p-cresol share the enzymes for the oxidation of para-methyl group in Pseudomonas putida NCIMB 9866 (2014), Appl. Microbiol. Biotechnol., 98, 1349-1356 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4-xylenol + acceptor + H2O Pseudomonas putida
-
4-hydroxy-3-methylbenzaldehyde + reduced acceptor
-
?
2,4-xylenol + acceptor + H2O Pseudomonas putida NCIMB 9866
-
4-hydroxy-3-methylbenzaldehyde + reduced acceptor
-
?
4-methylphenol + acceptor + H2O Pseudomonas putida
-
4-hydroxybenzaldehyde + reduced acceptor + H+
-
?
4-methylphenol + acceptor + H2O Pseudomonas putida NCIMB 9866
-
4-hydroxybenzaldehyde + reduced acceptor + H+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida NCIMB 9866
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-xylenol + acceptor + H2O
-
Pseudomonas putida 4-hydroxy-3-methylbenzaldehyde + reduced acceptor
-
?
2,4-xylenol + acceptor + H2O
-
Pseudomonas putida NCIMB 9866 4-hydroxy-3-methylbenzaldehyde + reduced acceptor
-
?
2,4-xylenol + phenazine methosulfate + H2O
-
Pseudomonas putida 4-hydroxy-3-methylbenzaldehyde + reduced phenazine methosulfate + H+
-
?
2,4-xylenol + phenazine methosulfate + H2O
-
Pseudomonas putida NCIMB 9866 4-hydroxy-3-methylbenzaldehyde + reduced phenazine methosulfate + H+
-
?
4-methylphenol + acceptor + H2O
-
Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor + H+
-
?
4-methylphenol + acceptor + H2O
-
Pseudomonas putida NCIMB 9866 4-hydroxybenzaldehyde + reduced acceptor + H+
-
?
4-methylphenol + phenazine methosulfate + H2O
-
Pseudomonas putida 4-hydroxybenzaldehyde + reduced phenazine methosulfate + H+
-
?
4-methylphenol + phenazine methosulfate + H2O
-
Pseudomonas putida NCIMB 9866 4-hydroxybenzaldehyde + reduced phenazine methosulfate + H+
-
?

Synonyms

Synonyms Comment Organism
p-cresol methylhydroxylase
-
Pseudomonas putida
pchC
-
Pseudomonas putida
pchF
-
Pseudomonas putida
PCMH
-
Pseudomonas putida

General Information

General Information Comment Organism
metabolism the enzyme is necessary for 2,4-xylenol catabolism Pseudomonas putida