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Literature summary for 1.17.9.1 extracted from

  • Joesaar, M.; Heinaru, E.; Viggor, S.; Vedler, E.; Heinaru, A.
    Diversity of the transcriptional regulation of the pch gene cluster in two indigenous p-cresol-degradative strains of Pseudomonas fluorescens (2010), FEMS Microbiol. Ecol., 72, 464-475.
    View publication on PubMed

Application

Application Comment Organism
analysis the enzyme is used as a tool to characterize catabolic differences between phenol- and p-cresol-degrading Pseudomonas fluorescens strains PC18 and PC24, overview Pseudomonas fluorescens

Cloned(Commentary)

Cloned (Comment) Organism
the pchACXF operon encodes p-hydroxybenzaldehyde dehydrogenase and PCMH, mapping of the transcriptional start sites of the pch operons, DNA and amino acid sequence determination and analysis, sequence comparisons, and phylogenetic analysis, gene pchF expression in Escherichia coli strain DH5alpha Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-methylphenol + acceptor + H2O Pseudomonas fluorescens
-
4-hydroxybenzaldehyde + reduced acceptor
-
?
4-methylphenol + acceptor + H2O Pseudomonas fluorescens PC24
-
4-hydroxybenzaldehyde + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens C6JUF6 gene pchF
-
Pseudomonas fluorescens C6JUG1 gene pchF
-
Pseudomonas fluorescens PC24 C6JUF6 gene pchF
-
Pseudomonas fluorescens PC24 C6JUG1 gene pchF
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylphenol + acceptor + H2O
-
Pseudomonas fluorescens 4-hydroxybenzaldehyde + reduced acceptor
-
?
4-methylphenol + acceptor + H2O
-
Pseudomonas fluorescens PC24 4-hydroxybenzaldehyde + reduced acceptor
-
?

Synonyms

Synonyms Comment Organism
p-cresolmethylhydroxylase
-
Pseudomonas fluorescens
PCMH
-
Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Pseudomonas fluorescens

Expression

Organism Comment Expression
Pseudomonas fluorescens different induction patterns of strains PC18 and PC24 are caused by differences in the PchR regulators of these strains additional information
Pseudomonas fluorescens PCMH of strain PC18 is induced during growth on phenol up

General Information

General Information Comment Organism
metabolism PCMH is a key enzyme responsible for the catabolism of p-cresol via the protocatechuate ortho pathway, pathway overview Pseudomonas fluorescens
additional information transcriptional regulation of the pchACXF operon by PchR, a putative sigma54-dependent regulator, overview. Phenol does not activate the regulator of PC24, and both regulators respond similarly to p-cresol Pseudomonas fluorescens
additional information transcriptional regulation of the pchACXF operon by PchR, a putative sigma54-dependent regulator, overview. Phenol is a more efficient effector for PchR of PC18 than p-cresol Pseudomonas fluorescens