Application | Comment | Organism |
---|---|---|
analysis | the enzyme is used as a tool to characterize catabolic differences between phenol- and p-cresol-degrading Pseudomonas fluorescens strains PC18 and PC24, overview | Pseudomonas fluorescens |
Cloned (Comment) | Organism |
---|---|
the pchACXF operon encodes p-hydroxybenzaldehyde dehydrogenase and PCMH, mapping of the transcriptional start sites of the pch operons, DNA and amino acid sequence determination and analysis, sequence comparisons, and phylogenetic analysis, gene pchF expression in Escherichia coli strain DH5alpha | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylphenol + acceptor + H2O | Pseudomonas fluorescens | - |
4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
4-methylphenol + acceptor + H2O | Pseudomonas fluorescens PC24 | - |
4-hydroxybenzaldehyde + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | C6JUF6 | gene pchF | - |
Pseudomonas fluorescens | C6JUG1 | gene pchF | - |
Pseudomonas fluorescens PC24 | C6JUF6 | gene pchF | - |
Pseudomonas fluorescens PC24 | C6JUG1 | gene pchF | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylphenol + acceptor + H2O | - |
Pseudomonas fluorescens | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
4-methylphenol + acceptor + H2O | - |
Pseudomonas fluorescens PC24 | 4-hydroxybenzaldehyde + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
p-cresolmethylhydroxylase | - |
Pseudomonas fluorescens |
PCMH | - |
Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas fluorescens |
Organism | Comment | Expression |
---|---|---|
Pseudomonas fluorescens | different induction patterns of strains PC18 and PC24 are caused by differences in the PchR regulators of these strains | additional information |
Pseudomonas fluorescens | PCMH of strain PC18 is induced during growth on phenol | up |
General Information | Comment | Organism |
---|---|---|
metabolism | PCMH is a key enzyme responsible for the catabolism of p-cresol via the protocatechuate ortho pathway, pathway overview | Pseudomonas fluorescens |
additional information | transcriptional regulation of the pchACXF operon by PchR, a putative sigma54-dependent regulator, overview. Phenol does not activate the regulator of PC24, and both regulators respond similarly to p-cresol | Pseudomonas fluorescens |
additional information | transcriptional regulation of the pchACXF operon by PchR, a putative sigma54-dependent regulator, overview. Phenol is a more efficient effector for PchR of PC18 than p-cresol | Pseudomonas fluorescens |