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Literature summary for 1.17.9.1 extracted from
- A study of the spectral and redox properties and covalent flavinylation of the flavoprotein component of p-cresol methylhydroxylase reconstituted with FAD analogues (2004), Biochemistry, 43, 10532-10546.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the flavoprotein subunit with non-covalently attached FAD in Escherichia coli | Pseudomonas putida |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y384F | strongly decreased activity | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 10000 | - |
alpha, beta, 2 * 58000 + 2 * 10000, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) | Pseudomonas putida |
| 58000 | - |
alpha, beta, 2 * 58000 + 2 * 10000, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-cresol + acceptor + H2O | Pseudomonas putida | via intermediate 4-hydroxybenzylalcohol | 4-hydroxybenzaldehyde + reduced acceptor | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzymes | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-cresol + acceptor + H2O | via intermediate 4-hydroxybenzylalcohol | Pseudomonas putida | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha, beta, 2 * 58000 + 2 * 10000, c-type cytochrome subunit PchC (beta) and two flavoprotein subunits PchF (alpha) | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | consists of a c-type cytochrome subunit PchC and two flavoprotein subunits PchF | Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.8 | - |
4-Cresol | Y384F mutant enzyme, pH 7, 25°C | Pseudomonas putida | |
| 121 | - |
4-Cresol | native enzyme, pH 7, 25°C | Pseudomonas putida | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Pseudomonas putida | |
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