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Literature summary for 1.17.9.1 extracted from

  • McIntire, W.; Hopper, D.J.; Singer, T.P.
    p-Cresol methylhydroxylase. assay and general properties (1985), Biochem. J., 228, 325-335.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.9.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information effect of pH-value and ionic strength on KM-value Pseudomonas putida
0.0264
-
 phenazine methosulfate reaction with 4-n-propylphenol Pseudomonas putida
0.366
-
 phenazine methosulfate reaction with 2-bromo-4-methylphenol Pseudomonas putida
1.94
-
 phenazine methosulfate reaction with 4-ethylphenol Pseudomonas putida
3.26
-
 phenazine methosulfate reaction with 4-hydroxybenzyl alcohol Pseudomonas putida
5.23
-
 phenazine methosulfate reaction with 2,4-dimethylphenol Pseudomonas putida
6.76
-
 phenazine methosulfate reaction with 4-cresol Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(RS)-1-(4-hydroxyphenyl)ethanol + acceptor + H2O
-
 Pseudomonas putida ?
-
 ?
1,4-hydroxyquinone + acceptor + H2O
-
 Pseudomonas putida ?
-
 ?
2,4-xylenol + acceptor + H2O i.e. 2,4-dimethylphenol Pseudomonas putida 3-methyl-4-hydroxybenzaldehyde + reduced acceptor
-
 ?
2-bromo-4-methylphenol + acceptor + H2O
-
 Pseudomonas putida 3-bromo-4-hydroxybenzaldehyde + reduced acceptor
-
 ?
2-methoxy-4-methylphenol + acceptor + H2O
-
 Pseudomonas putida 3-methoxy-4-hydroxybenzaldehyde + reduced acceptor
-
 ?
3,4-dimethylphenol + acceptor + H2O
-
 Pseudomonas putida 2-methyl-4-hydroxybenzaldehyde + reduced acceptor
-
 ?
3-fluoro-4-methylphenol + acceptor + H2O
-
 Pseudomonas putida 2-fluoro-4-hydroxybenzaldehyde + reduced acceptor
-
 ?
4-cresol + acceptor + H2O acceptor: phenazine methosulfate Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
 ?
4-cresol + acceptor + H2O no activity with 2,6-dichlorophenol-indophenol as acceptor Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
 ?
4-cresol + acceptor + H2O no activity with O2, K3Fe(CN)6 or methylene blue Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
 ?
4-cresol + acceptor + H2O the enzyme catalyzes both 4-cresol hydroxylation and further oxidation of the product, 4-hydroxybenzyl alcohol to 4-hydroxybenzaldehyde Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
 ?
4-cresol + phenazine methosulfate + H2O
-
 Pseudomonas putida 4-hydroxybenzyl alcohol + reduced phenazine methosulfate
-
 ?
4-ethylphenol + acceptor + H2O
-
 Pseudomonas putida 1-(4'-hydroxyphenyl)ethanol + reduced acceptor
-
 ?
4-hydroxybenzyl alcohol + acceptor
-
 Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
 ?
4-isopropylphenol + acceptor + H2O
-
 Pseudomonas putida ?
-
 ?
4-methyl-1-naphthol + acceptor + H2O
-
 Pseudomonas putida ?
-
 ?
4-methyl-3-nitrophenol + acceptor + H2O
-
 Pseudomonas putida 2-nitro-4-hydroxybenzaldehyde + reduced acceptor
-
 ?
4-methylcatechol + acceptor + H2O
-
 Pseudomonas putida 3,4-dihydroxybenzaldehyde + reduced acceptor
-
 ?
4-n-propylphenol + acceptor + H2O
-
 Pseudomonas putida ?
-
 ?
additional information no activity with 2-cresol Pseudomonas putida ?
-
 ?
additional information no activity with 4-methyl-2-nitrophenol, 2,4,5-trimethylphenol, 2-chloro-4,5-dimethylphenol, 2,4,6-trimethylphenol, 2,6-dibromo-4-methylphenol, L-tyrosine, 4-hydroxybenzaldehyde 3-cresol Pseudomonas putida ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
cytochrome c enzyme contains covalently bound flavin and a type c cytochrome Pseudomonas putida